State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
J Agric Food Chem. 2022 May 25;70(20):6202-6212. doi: 10.1021/acs.jafc.2c00152. Epub 2022 May 12.
Glycogen-like glucan (GnG) is a unique hyperbranched polysaccharide nanoparticle which is drawing increasing attention due to its biodegradability and abundant short branches that can be functionalized. Because starch and GnG are both composed of glucose residues and have similar glucosidic bonds, GnG could be fabricated by sucrose phosphorylase, α-glucan phosphorylase, and branching enzymes from starch primers and sucrose. In this study, high-amylose starch, normal starch, and waxy corn starch were used as primers to synthesize GnG, and their impact on the fine structure of GnG was investigated. Structural analysis indicated that with increasing content of amylopectin in the starch primer, the proportion of short chains in GnG decreased, and the degree of β-amylolysis and α-amylolysis was enhanced. Amylose in the primer contributed to a compact and homogeneous structure of GnG, while amylopectin triggered the formation of branch points with a more open distribution. These findings provide a new strategy for regulating the fine structure of GnG.
糖原样葡聚糖 (GnG) 是一种独特的超支化多糖纳米颗粒，由于其可生物降解性和丰富的短支链可以被功能化，因此引起了越来越多的关注。由于淀粉和 GnG 都是由葡萄糖残基组成，并且具有相似的糖苷键，因此 GnG 可以由蔗糖磷酸化酶、α-葡聚糖磷酸化酶和支链酶从淀粉引物和蔗糖中合成。在本研究中，高直链淀粉淀粉、普通淀粉和蜡质玉米淀粉被用作引物来合成 GnG，并研究了它们对 GnG 精细结构的影响。结构分析表明，随着淀粉引物中支链淀粉含量的增加，GnG 中短链的比例降低，β-淀粉酶解和α-淀粉酶解程度增强。引物中的直链淀粉有助于 GnG 形成紧凑和均匀的结构，而支链淀粉引发具有更开放分布的分支点形成。这些发现为调节 GnG 的精细结构提供了一种新策略。
