Hadjimi Zohra, Belguendouz Houda, Benchabane Sarah, Ghozali Nour El Houda, Amri Manel, Kocheida Racha, Hakem Djenette, Biad Ahmed, Otmani Fifi, Kemikem Yasmine, Terahi Malika, Touil-Boukoffa Chafia
Laboratory of Cellular and Molecular Biology (LBCM), Cytokines and NO Synthases Group, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), Algiers, Algeria.
Dentistry Department, Isaad Hassani Beni Messous hospital, Algiers, Algeria.
Endocr Metab Immune Disord Drug Targets. 2023;23(3):347-355. doi: 10.2174/1871530322666220512120948.
Oral aphthosis is one of the major manifestations of Behçet's disease (BD), a chronic, multisystemic vasculitis. BD etio-pathogenicity related to oral health lack.
This study investigated the possible relationships between poor oral hygiene, oral activity, disease severity and saliva's Interleukin (IL)-32, IL-6, IL-10 and nitric oxide (NO) levels in Behçet's patients to determine their role in disease prognosis and their potential therapeutic interest.
Fifty-six patients with BD (22 orally active; 34 orally inactive) and 31 healthy subjects have been included in our study. Salivary levels of IL-32, IL-6, and IL-10 were measured using ELISA, while NO levels were assessed by modified Griess's method. Oral health status and disease severity scores were recorded for each participant. Kruskal-Wallis test and Spearman's test were performed for statistical analyses.
We observed that the tested molecules were increased in BD patients compared to healthy controls (p˂0.05). Moreover, only IL-32 levels were associated with oral activity in patients (p˂0.05). Interestingly, the disease severity score was noted to be correlated positively and significantly with both IL-32 saliva levels (p˂0.01) and plaque index (p˂0.05) in BD patients. Furthermore, IL-32 levels were correlated with plaque index (p˂0.0001).
Our results suggest that IL-32, IL- 6, IL-10 and NO were increased in saliva during BD. Our study indicated that IL-32 was associated with the genesis of oral ulcers in response to dental plaque. Ultimately, salivary IL-32 may serve as a prognostic biomarker and a possible therapeutic target for managing Behçet's disease severity.
口腔阿弗他病是白塞病（BD）的主要表现之一，白塞病是一种慢性多系统血管炎。BD的病因与口腔健康缺乏有关。
本研究调查了白塞病患者口腔卫生不良、口腔活动、疾病严重程度与唾液白细胞介素（IL）-32、IL-6、IL-10和一氧化氮（NO）水平之间的可能关系，以确定它们在疾病预后中的作用及其潜在的治疗意义。
本研究纳入了56例BD患者（22例口腔活动者；34例口腔不活动者）和31名健康受试者。采用酶联免疫吸附测定法（ELISA）检测唾液中IL-32、IL-6和IL-10的水平，同时采用改良的格里斯方法评估NO水平。记录每位参与者的口腔健康状况和疾病严重程度评分。采用Kruskal-Wallis检验和Spearman检验进行统计分析。
我们观察到，与健康对照组相比，BD患者中检测的分子水平升高（p<0.05）。此外，患者中只有IL-32水平与口腔活动相关（p<0.05）。有趣的是，在BD患者中，疾病严重程度评分与唾液IL-32水平（p<0.01）和菌斑指数（p<0.05）均呈显著正相关。此外，IL-32水平与菌斑指数相关（p<0.0001）。
我们的结果表明，BD患者唾液中IL-32、IL-6、IL-10和NO水平升高。我们的研究表明，IL-32与牙菌斑引起的口腔溃疡的发生有关。最终，唾液IL-32可能作为一种预后生物标志物和控制白塞病严重程度的潜在治疗靶点。
