State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
State Key Laboratory of Natural Medicines, School of Engineering, China Pharmaceutical University, Nanjing 210009, China.
Res Microbiol. 2022 Jul-Sep;173(6-7):103955. doi: 10.1016/j.resmic.2022.103955. Epub 2022 May 10.
Candida albicans can form biofilm on biotic and abiotic surfaces of medical implants to cause superficial and systemic infections under specific condition. The formation of hyphae and matrix of C. albicans are considered as probable virulence factors. We assessed the inhibitory activities of 26 anthraquinones against C. albicans biofilm formation, which were substituted by different functional groups including hydroxyl groups, amino groups, carboxyl groups, alkyl groups, and glycoside groups at C1- or C2-position. Among them, anthraquinones without substituents at other positions but only an alkyl group attached to C2-position, namely 2-alkyl-anthraquinones were determined to have significant anti-biofilm activities. Furthermore, 2-ethylanthraquinone can significantly affect genes related to extracellular matrix (PMT6 and IFD6), and hyphal formation (HWP1, ECE1 and EFG1), leading to the disrupted formation of biofilm, by detail transcriptomics analysis. We believed that 2-ethylanthraquinone could inspire more discoveries of anti-biofilm agents against C. albicans.
白色念珠菌可以在医学植入物的生物和非生物表面形成生物膜，在特定条件下会引起浅表和全身感染。菌丝和白色念珠菌基质的形成被认为是可能的毒力因素。我们评估了 26 种蒽醌类化合物对白色念珠菌生物膜形成的抑制活性，这些化合物在 C1 或 C2 位被不同的官能团取代，包括羟基、氨基、羧基、烷基和糖苷基。其中，在其他位置没有取代基但在 C2 位只有一个烷基的蒽醌类化合物，即 2-烷基蒽醌类化合物，被确定具有显著的抗生物膜活性。此外，通过详细的转录组学分析，2-乙基蒽醌可以显著影响与细胞外基质（PMT6 和 IFD6）和菌丝形成（HWP1、ECE1 和 EFG1）相关的基因，导致生物膜形成的破坏。我们相信，2-乙基蒽醌可以激发更多针对白色念珠菌的抗生物膜剂的发现。
