软砂岩地区土壤 AM 真菌群落的分布特征。
Distribution characteristics of soil AM fungi community in soft sandstone area.
机构信息
MOE Key Laboratory of Thermo-Fluid Science and Engineering, Xi'an Jiaotong University, Xi'an, 710049, China; School of Life Sciences, Inner Mongolia University, Hohhot, 010018, China; State Key Laboratory of Water Resource Protection and Utilization in Coal Mining, Beijing, 100000, China; China Energy Group, Shendong Coal Group Technology Research Institute, Ordos, 017200, China.
School of Life Sciences, Inner Mongolia University, Hohhot, 010018, China.
出版信息
J Environ Manage. 2022 Aug 15;316:115193. doi: 10.1016/j.jenvman.2022.115193. Epub 2022 May 9.
To explore the diversity and distribution characteristics of soil arbuscular mycorrhizae fungi (AMF) communities in the soft sandstone area, thirteen arsenic sandstone rock samples were collected from three planting plots (SI, SII and SIII) and one bare control plot (CK), separately. The sampling locations are as follows: the top of the slope (denoted by the number 1), sunny slope (2), shady slope (3) and gully bottom (4). These samples were then tested with an Illumina HiSeq PE250 high-throughput sequencing platform. Experimental results show that the SIII4 sample (from the gully bottom of the SIII plot) has the highest moisture content of 9.1%, while the CK sample in the control plot has lowest moisture content. SI2 has the highest pH of 9.58 and CK has the lowest pH of 8.73. SII1 has the highest available phosphorus (AP) content of 9.61 mg/kg, while SII3 has the lowest AP content of 2.29 mg/kg. Furthermore, SI2 has the highest NH-N content of 11.24 mg/kg, while SII1 has the lowest NH-N of 4.09 mg/kg. SII1 has the highest available potassium (AK) content of 48.92 mg/kg and CK has the lowest AK content of 1.82 mg/kg. In the observed-species index reflecting AMF genetic diversity, SI1 differences significantly from SII4 and SIII3 (P < 0.05). In the Shannon index, SI1 is significantly different from SI2, SI3, SI4; SII2 is significantly different from SII3; SI2, SI4, SII1 and SII3 are quite different from CK (P < 0.05). The dominant genera of AMF in these plots include Glomus (17.24%-65.53%), Scutellospora (0.04%-67.38%), Septoglomus (2.83%-43.03%) and Kamienskia (0.64%-46.38%). The dominant genera of AMF vary significantly between sunny slope and shady slope. Positive correlation exists between soil NH-N and the AM fungal community structure. There are prominent positive correlations exist among genetic diversity index chao1, observed-species, pH and AP (P < 0.05), and obviously negative correlation between observed species and AK (P < 0.05). The research findings on the distribution characteristics of AM fungus community in the arsenic sandstone plot and their relationship with environmental factors can help with arsenic sandstone management in other similar areas.
为了探究软砂岩地区土壤丛枝菌根真菌（AMF）群落的多样性和分布特征，从三个种植区（SI、SII 和 SIII）和一个对照区（CK）的 13 个砷砂岩岩样中采集了土壤样本。采样点分别位于：坡顶（编号 1）、阳坡（2）、阴坡（3）和沟底（4）。然后，这些样本使用 Illumina HiSeq PE250 高通量测序平台进行了测试。实验结果表明，SIII4 样本（来自 SIII 区的沟底）的含水量最高，为 9.1%，而对照区 CK 的含水量最低。SI2 的 pH 值最高，为 9.58，而 CK 的 pH 值最低，为 8.73。SII1 的有效磷（AP）含量最高，为 9.61mg/kg，而 SII3 的 AP 含量最低，为 2.29mg/kg。此外，SI2 的 NH-N 含量最高，为 11.24mg/kg，而 SII1 的 NH-N 含量最低，为 4.09mg/kg。SII1 的速效钾（AK）含量最高，为 48.92mg/kg，CK 的 AK 含量最低，为 1.82mg/kg。在反映 AMF 遗传多样性的观测种指数中，SI1 与 SII4 和 SIII3 差异显著（P<0.05）。在 Shannon 指数中，SI1 与 SI2、SI3、SI4 差异显著；SII2 与 SII3 差异显著；SI2、SI4、SII1 和 SII3 与 CK 差异显著（P<0.05）。这些地块中 AMF 的优势属包括 Glomus（17.24%-65.53%）、Scutellospora（0.04%-67.38%）、Septoglomus（2.83%-43.03%）和 Kamienskia（0.64%-46.38%）。阳坡和阴坡的 AMF 优势属存在显著差异。土壤 NH-N 与 AM 真菌群落结构呈正相关。Chao1、观测种、pH 和 AP 之间存在显著正相关（P<0.05），而观测种与 AK 之间存在明显负相关（P<0.05）。对砷砂岩区 AM 真菌群落分布特征及其与环境因子关系的研究结果，有助于对其他类似地区的砷砂岩进行管理。
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