Chen Fen, Yu Gao, Wang Xie-Feng, Li Ting-Liang, Sun Yue-Bing
Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region, Tongren University, Tongren 554300, China.
College of Resources and Environment, Shanxi Agricultural University, Jinzhong 030801, China.
Huan Jing Ke Xue. 2024 Jan 8;45(1):543-554. doi: 10.13227/j.hjkx.202303078.
This study aimed to clarify the effect of long-term continuous cropping of pepper on soil fungal community structure, reveal the mechanism of continuous cropping obstacles, and provide a theoretical basis for the ecological safety and sustainable development of pepper industry. We took the pepper continuous cropping soil in the vegetable greenhouse planting base of Tongren City as the research object. The diversity and community structure of fungi in farmland soil were analyzed using Illumina MiSeq high-throughput sequencing, the responses of soil physio-chemical properties and fungal community characteristics to long-term continuous pepper cropping were discussed, and the relationships between the characteristics of fungal community structure and environmental factors were determined using CCA and correlation network analysis. The results showed that with the extension of pepper continuous cropping years, the soil pH value and organic matter (OM) content decreased, total phosphorus (TP) and available phosphorus (AP) contents increased, hydrolyzed nitrogen (AN) and available potassium (AK) contents decreased first and then increased, and total nitrogen (TN) and total potassium (TK) contents did not change significantly. Long-term continuous cropping decreased the Chao1 index and observed species index and decreased the Shannon index and Simpson index. The change in continuous cropping years had a significant effect on the relative abundance of soil fungal dominant flora. At the phylum level, the relative abundance of Mortierellomycota decreased with the extension of pepper continuous cropping years, the relative abundance of Ascomycota increased first and then decreased, and the relative abundance of Basidiomycota decreased first and then increased. At the genus level, with the increasing of pepper continuous cropping years, the relative abundance of increased, and the relative abundance of and decreased. In addition, long-term continuous cropping simplified the soil fungal symbiosis network. CCA analysis indicated that pH, OM, TN, AN, AP, and AK were the driving factors of soil fungal community structure, and correlation network analysis showed that pH, OM, TN, TP, TK, AN, AP, and AK were the driving factors of soil fungal community structure, including , , , , , , , and . In conclusion, continuous cropping changed the soil physical and chemical properties, affected the diversity and community structure of the soil fungal community, changed the interaction between microorganisms, and destroyed the microecological balance of the soil, which might explain obstacles associated with continuous cropped pepper.
本研究旨在阐明辣椒长期连作对土壤真菌群落结构的影响，揭示连作障碍机制，为辣椒产业的生态安全和可持续发展提供理论依据。我们以铜仁市蔬菜大棚种植基地的辣椒连作土壤为研究对象。采用Illumina MiSeq高通量测序分析农田土壤真菌的多样性和群落结构，探讨土壤理化性质和真菌群落特征对辣椒长期连作的响应，并利用CCA和相关网络分析确定真菌群落结构特征与环境因子之间的关系。结果表明，随着辣椒连作年限的延长，土壤pH值和有机质（OM）含量降低，总磷（TP）和有效磷（AP）含量增加，水解氮（AN）和速效钾（AK）含量先降低后增加，总氮（TN）和总钾（TK）含量变化不显著。长期连作降低了Chao1指数和观测物种指数，降低了Shannon指数和Simpson指数。连作年限的变化对土壤真菌优势菌群的相对丰度有显著影响。在门水平上，被孢霉门的相对丰度随着辣椒连作年限的延长而降低，子囊菌门的相对丰度先增加后降低，担子菌门的相对丰度先降低后增加。在属水平上，随着辣椒连作年限的增加， 的相对丰度增加， 和 的相对丰度降低。此外，长期连作简化了土壤真菌共生网络。CCA分析表明，pH、OM、TN、AN、AP和AK是土壤真菌群落结构的驱动因素，相关网络分析表明，pH、OM、TN、TP、TK AN、AP和AK是土壤真菌群落结构的驱动因素，包括 , , , , , , , 和 。总之，连作改变了土壤理化性质，影响了土壤真菌群落的多样性和群落结构，改变了微生物之间的相互作用，破坏了土壤微生态平衡，这可能解释了辣椒连作相关的障碍。
