Institute of Protein Research, RAS, Pushchino, Moscow Region, Russia.
PLoS One. 2022 May 12;17(5):e0265683. doi: 10.1371/journal.pone.0265683. eCollection 2022.
Pseudomonas syringae is a widely spread plant pathogen known to have ice-nucleating proteins that serve as crystallization sites promoting ice growth at near-zero temperatures. Three temperatures that characterize water freezing and ice melting are (i) the freezing point of water, (ii) the temperature of coexistence of ice and water, and (iii) the melting point of ice. Here we show the influence of different concentrations of P. syringae on these three parameters. P. syringae appears to affect both the freezing point of water and the temperature of the coexistence of ice and water. Additionally, we propose a research technique for studying the freezing/melting process that is simple and requires no complex equipment.
丁香假单胞菌是一种广泛分布的植物病原体,已知其具有冰核蛋白,可作为促进近零温度下冰生长的结晶位点。有三个温度特征可以描述水的冻结和冰的融化:(i)水的冰点,(ii)冰和水共存的温度,以及(iii)冰的熔点。在这里,我们展示了不同浓度的丁香假单胞菌对这三个参数的影响。丁香假单胞菌似乎同时影响水的冰点和冰与水共存的温度。此外,我们提出了一种简单且不需要复杂设备的研究冻结/融化过程的研究技术。