Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Center for Infection and Immunity of Lille, 59000, Lille, France.
Univ. Lille, CNRS, Centrale Lille, Univ. Artois, UMR 8181, UCCS, Unité de Catalyse et Chimie du Solide, 59000, Lille, France.
Angew Chem Int Ed Engl. 2022 Jul 18;61(29):e202204992. doi: 10.1002/anie.202204992. Epub 2022 May 31.
We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable reagents, they can be activated on demand to enable ligation reactions with peptide concentrations as low as a few hundred nM at rates approaching 30 M s .
我们证明了潜伏的草酰硫代酯类似物是在高度稀释的条件下在纯化的水介质中或在复杂如细胞裂解物的混合物中修饰肽和蛋白质的有效手段。作为货架稳定试剂设计，它们可以按需激活，以实现与肽浓度低至几百 nM 的连接反应，接近 30 M s 的速率。
