Hornby P J, Piekut D T
Cell Tissue Res. 1987 May;248(2):239-46. doi: 10.1007/BF00218190.
The distribution of catecholamine synthesizing enzymes within the paraventricular nucleus of the rat hypothalamus is elucidated immunocytochemically by use of antibodies to tyrosine hydroxylase, dopamine beta-hydroxylase, and phenylethanolamine-N-methyltransferase. Tyrosine hydroxylase-immunostained cell bodies are localized in the periventricular stratum and adjacent parvocellular regions, but rarely in magnocellular subnuclei of the paraventricular nucleus. Tyrosine hydroxylase-immunostained fibers are present in greatest density in the periventricular zone, and moderate density in the parvocellular and magnocellular subnuclei. Dopamine beta-hydroxylase-immunostained fibers are remarkably dense in the posterior magnocellular division of the paraventricular nucleus, especially in the dorso-lateral portion where vasopressin-containing cells predominate. Noradrenergic fiber input to these magnocellular neurons is likely since phenylethanolamine-N-methyltransferase-immunostained fibers are sparse in magnocellular subnuclei of the paraventricular nucleus. Dual immunocytochemical staining of thick and thin tissue sections demonstrates with clarity an anatomical association of dopamine beta-hydroxylase-immunostained fibers and magnocellular neurons. Dopamine beta-hydroxylase-immunostained and phenylethanolamine-N-methyltransferase-immunostained fibers are dense in the medial parvocellular component of the paraventricular nucleus; distinct features of both antisera are presented.
利用针对酪氨酸羟化酶、多巴胺β-羟化酶和苯乙醇胺-N-甲基转移酶的抗体，通过免疫细胞化学方法阐明了大鼠下丘脑室旁核内儿茶酚胺合成酶的分布情况。酪氨酸羟化酶免疫染色的细胞体位于室周层和相邻的小细胞区域，但很少见于室旁核的大细胞亚核。酪氨酸羟化酶免疫染色的纤维在室周区密度最高，在小细胞和大细胞亚核中密度中等。多巴胺β-羟化酶免疫染色的纤维在室旁核的后大细胞区显著密集，尤其是在含血管加压素细胞占主导的背外侧部分。由于苯乙醇胺-N-甲基转移酶免疫染色的纤维在室旁核的大细胞亚核中稀疏，因此这些大细胞神经元可能有去甲肾上腺素能纤维输入。对厚组织切片和薄组织切片进行双重免疫细胞化学染色，清晰地显示了多巴胺β-羟化酶免疫染色的纤维与大细胞神经元之间的解剖学关联。多巴胺β-羟化酶免疫染色和苯乙醇胺-N-甲基转移酶免疫染色的纤维在室旁核的内侧小细胞成分中密集；呈现了两种抗血清的独特特征。
