C10orf91和LINC01224在肝细胞癌中的高表达与不良预后相关。
High expression of C10orf91 and LINC01224 in hepatocellular carcinoma and poor prognosis.
作者信息
Gong Anan, Luo Xuhang, Tan Yiwei, Chen Hao, Luo Gaojian
机构信息
Yiwu Central Hospital No. 699 Jiangdong Road, Yiwu 322000, Zhejiang Province, China.
出版信息
Am J Transl Res. 2022 Apr 15;14(4):2567-2579. eCollection 2022.
BACKGROUND
The dysregulation of long non-coding RNAs (lncRNAs) has been implicated roles in the pathogenesis of many human diseases, including hepatic diseases. Several lncRNAs have been associated with the progression of hepatocellular carcinoma (HCC), but their function as diagnostic markers for liver cancer remain to be determined.
OBJECTIVE
This study aimed to identify the potential diagnostic markers for liver cancer.
METHODS
The Cancer Genome Atlas (TCGA) database was used to obtain the gene transcriptome data of liver cancer. In addition, this study enrolled 70 liver cancer patients admitted to the Yiwu Central Hospital and 50 healthy people who concurrently underwent physical examinations from February 2017 to January 2020. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the expression of C10orf91 and LINC01224 in the patients' tissues and serum. A 5-year follow-up was conducted for survival observation. The potential and targeted miRs of C10orf91 and LINC01224 were predicted by online database for miRNA target prediction. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted and competing endogenous RNA (ceRNA) network was plotted.
RESULTS
A total of 175 differentially expressed lncRNAs were screened out, of which 173 were upregulated and 2 were downregulated. C10orf91, and LINC01224 were independent prognostic factors for liver cancer (P<0.05). C10orf91 and LINC01224 had diagnostic value for differentiating liver cancer, tumor node metastasis (TNM) staging, and lymphatic metastasis. GO and KEGG enrichment analysis showed that C10orf91 and LINC01224 were involved in 23 significant biological functions and 35 significant signal transduction pathways respectively.
CONCLUSION
C10orf91 and LINC01224 are highly expressed in liver cancer patients withpoor prognosis.
背景
长链非编码RNA（lncRNAs）失调在包括肝脏疾病在内的多种人类疾病发病机制中发挥作用。一些lncRNAs与肝细胞癌（HCC）进展相关，但其作为肝癌诊断标志物的功能仍有待确定。
目的
本研究旨在鉴定肝癌潜在诊断标志物。
方法
利用癌症基因组图谱（TCGA）数据库获取肝癌基因转录组数据。此外，本研究纳入2017年2月至2020年1月在义乌市中心医院收治的70例肝癌患者和同期进行体检的50名健康人。采用定量逆转录聚合酶链反应（qRT-PCR）检测患者组织和血清中C10orf91和LINC01224的表达。进行为期5年的生存观察随访。通过在线miRNA靶标预测数据库预测C10orf91和LINC01224的潜在及靶向miRs。进行基因本体（GO）和京都基因与基因组百科全书（KEGG）富集分析并绘制竞争性内源性RNA（ceRNA）网络。
结果
共筛选出175个差异表达lncRNAs，其中173个上调，2个下调。C10orf91和LINC01224是肝癌独立预后因素（P<0.05）。C10orf91和LINC01224对鉴别肝癌、肿瘤淋巴结转移（TNM）分期及淋巴转移具有诊断价值。GO和KEGG富集分析表明，C10orf91和LINC01224分别参与23项显著生物学功能和35条显著信号转导通路。
结论
C10orf91和LINC01224在预后不良的肝癌患者中高表达。
Zotero 插件