Department of Otolaryngology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
J Cell Biochem. 2019 Aug;120(8):13202-13215. doi: 10.1002/jcb.28594. Epub 2019 Mar 19.
Esophageal squamous cell carcinoma (ESCC) is the predominant form with the highest incidence. We aimed to find metastasis-related differentially expressed long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and messenger RNA (mRNAs) in ESCC. We first obtained the lncRNAs, miRNAs, and mRNAs profiles. The differentially expressed lncRNAs, miRNAs, and mRNAs were obtained, followed by the functional annotation. Then the interaction networks of miRNA-mRNA, lncRNA-mRNA coexpression, lncRNA-miRNA, and lncRNA-miRNA-mRNA were constructed. In addition, systematic expression pattern analysis of differentially expressed lncRNAs, miRNA, and mRNA in the normal, metastasis, and nonmetastasis was performed. Survivability of differentially expressed lncRNAs, miRNAs, and mRNA was analyzed. A total of 613 differentially expressed lncRNAs, 35 differentially expressed miRNAs, and 1586 differentially expressed mRNAs were obtained. Several interactions of H19-hsa-mir-222-chromobox 2 (CBX2), H19-hsa-mir-330-phosphoinositide-3-kinase regulatory subunit 4 (PIK3R4), KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1)/CTB-89H12.4-hsa-mir-374a-vascular endothelial growth factor A (VEGFA), MALAT1/X inactive specific transcript (XIST)/XIST antisense RNA (TSIX)-hsa-mir-340-tumor necrosis factor receptor superfamily member 10A (NFRSF10A) were identified to play key roles in the metastasis of ESCC. In addition, KCNQ1OT1, TSIX, and XIST were significantly associated with the survival time of patients. In conclusion, our study may be helpful in understanding the pathological mechanism and providing new diagnostic and therapeutic biomarkers for ESCC.
食管鳞状细胞癌 (ESCC) 是最主要的形式，发病率最高。我们旨在寻找 ESCC 转移相关的差异表达长非编码 RNA (lncRNA)、微小 RNA (miRNA) 和信使 RNA (mRNA)。我们首先获得 lncRNA、miRNA 和 mRNA 谱。获得差异表达的 lncRNA、miRNA 和 mRNA，然后进行功能注释。然后构建 miRNA-mRNA、lncRNA-mRNA 共表达、lncRNA-miRNA 和 lncRNA-miRNA-mRNA 的相互作用网络。此外，对正常、转移和非转移组织中差异表达的 lncRNA、miRNA 和 mRNA 的系统表达模式进行分析。分析差异表达的 lncRNA、miRNA 和 mRNA 的存活率。共获得 613 个差异表达的 lncRNA、35 个差异表达的 miRNA 和 1586 个差异表达的 mRNA。鉴定了 H19-hsa-mir-222-盒 2 (CBX2)、H19-hsa-mir-330-磷酸肌醇 3-激酶调节亚基 4 (PIK3R4)、KCNQ1 反义/反义转录物 1 (KCNQ1OT1)/CTB-89H12.4-hsa-mir-374a-血管内皮生长因子 A (VEGFA)、MALAT1/X 失活特异性转录物 (XIST)/XIST 反义 RNA (TSIX)-hsa-mir-340-肿瘤坏死因子受体超家族成员 10A (NFRSF10A) 的相互作用，这些相互作用在 ESCC 的转移中起关键作用。此外，KCNQ1OT1、TSIX 和 XIST 与患者的生存时间显著相关。总之，我们的研究可能有助于理解病理机制，并为 ESCC 提供新的诊断和治疗生物标志物。
