Clinical and Experimental Endocrinology, Department of Anatomy, Unit for Multidisciplinary Research in Biomedicine, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal.
Clinical and Experimental Endocrinology, Department of Anatomy, Unit for Multidisciplinary Research in Biomedicine, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal; Química Orgânica, Produtos Naturais e Agroalimentares (QOPNA) and Laboratório Associado para a Química Verde (LAQV), Department of Chemistry, University of Aveiro, Portugal.
F S Sci. 2021 May;2(2):164-175. doi: 10.1016/j.xfss.2021.03.004. Epub 2021 Mar 26.
To study the abundance of obesity-related gene (ORG) mRNA in human spermatozoa and its association with sperm quality parameters, embryonic development, and pregnancy rates after assisted reproduction treatment (ART).
Cross-sectional study of spermatozoa ORG mRNA expression, and sperm and embryonic development parameters of infertile couples attending a single ART center.
University, in collaboration with a medically assisted reproduction center.
PATIENT(S): One hundred six couples seeking fertility treatment and receiving ART.
INTERVENTION(S): Expression of spermatozoa ORG mRNA was assessed by quantitative reverse transcription-polymerase chain reaction. Sperm and embryonic development parameters were measured by board-certified embryologists. Serum β-human chorionic gonadotropin levels and fetal heartbeat detection on ultrasound were used to document biochemical and clinical pregnancy, respectively.
MAIN OUTCOME MEASURE(S): Correlations between the abundance of ORG transcripts in spermatozoa and sperm quality, embryonic development, and achievement of pregnancy.
The abundance of spermatozoa FTO mRNA was positively correlated with total sperm count (r = 0.5030), fertilization rate (r = 0.4854), embryo cleavage rate (r = 0.5705), and high-quality embryo rate (r = 0.6982). The abundance of spermatozoa MC4R transcript was negatively correlated with sperm viability (r = -0.3111) and positively correlated with biochemical pregnancy (r = 0.4420). The abundance of MC4R and GNPDA2 transcripts was higher in spermatozoa of men with asthenozoospermia and teratozoospermia than in those with normozoospermia.
To our knowledge, this is the first report showing that the abundance of MC4R and FTO transcripts in spermatozoa is associated with sperm and embryo quality parameters, as well as pregnancy rates. Overall, these results further support the view that male factors beyond classic sperm quality parameters, namely the abundance of ORG transcripts, also affect the outcome of ART.
研究肥胖相关基因（ORG）mRNA 在人精子中的丰度及其与辅助生殖治疗（ART）后精子质量参数、胚胎发育和妊娠率的关系。
对在单一 ART 中心就诊的不孕夫妇的精子 ORG mRNA 表达以及精子和胚胎发育参数进行的横断面研究。
大学，与一家医学辅助生殖中心合作。
106 对寻求生育治疗并接受 ART 的夫妇。
通过定量逆转录-聚合酶链反应评估精子 ORG mRNA 的表达。由具有董事会认证的胚胎学家测量精子和胚胎发育参数。血清β-人绒毛膜促性腺激素水平和超声检测胎儿心跳分别用于记录生化妊娠和临床妊娠。
精子中 ORG 转录本丰度与精子质量、胚胎发育和妊娠的相关性。
精子 FTO mRNA 的丰度与总精子计数（r = 0.5030）、受精率（r = 0.4854）、胚胎卵裂率（r = 0.5705）和优质胚胎率（r = 0.6982）呈正相关。精子 MC4R 转录本的丰度与精子活力呈负相关（r = -0.3111），与生化妊娠呈正相关（r = 0.4420）。在弱精子症和畸形精子症患者的精子中，MC4R 和 GNPDA2 转录本的丰度高于正常精子症患者。
据我们所知，这是第一项表明精子中 MC4R 和 FTO 转录本的丰度与精子和胚胎质量参数以及妊娠率相关的报告。总的来说，这些结果进一步支持了这样一种观点，即除了经典的精子质量参数之外，男性因素，即 ORG 转录本的丰度，也会影响 ART 的结果。
