Department of Physics, Florida Atlantic University, Boca Raton, Florida; Asghar Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida.
Department of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida; Asghar Lab, Micro and Nanotechnology in Medicine, College of Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida.
F S Sci. 2021 Nov;2(4):376-382. doi: 10.1016/j.xfss.2021.10.003. Epub 2021 Oct 20.
To investigate whether the presented rheotaxis-based microfluidic device could be used to separate spermatozoa from viruses (i.e., Zika) in the infected semen sample during the selection and washing process.
Quantitative and experimental study of the sperm washing/selection process through the microfluidic platform exploiting the positive rheotaxis of sperm.
None.
PATIENT(S): None.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Human sperm were purchased from a sperm bank. The raw semen sample was mixed with viruses and loaded into a microfluidic device. Experiments were performed with 2 different flow rates (0 and 25 μL/minute) to investigate the washing efficiency of the device in the sperm selection process. The sperm sample was collected after 45 minutes and analyzed to check whether the collected sample is free of any infections (viruses) after isolation.
RESULT(S): Fluorescent microscopy and quantitative polymerase chain reaction-based analysis showed that the sperm selected with the presented rheotaxis-based microfluidic device at the optimal flow rate (25 μL/minute) was free of any viruses.
CONCLUSION(S): We have developed a simple, cost-effective microfluidic device that mimics the conditions of the female genital tract while washing out the raw semen efficiently during the selection process for assisted reproductive technology.
研究所提出的基于趋流性的微流控装置是否可用于在选择和洗涤过程中从感染精液样本中的精子中分离病毒（例如 Zika）。
通过利用精子的正趋流性的微流控平台对精子洗涤/选择过程进行定量和实验研究。
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无。
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从精子库购买人精子。将原始精液样本与病毒混合并加载到微流控装置中。以 0 和 25 μL/min 的 2 种不同流速进行实验，以研究该装置在精子选择过程中的洗涤效率。45 分钟后收集精子样本，并分析以检查在隔离后收集的样本是否没有任何感染（病毒）。
荧光显微镜和基于聚合酶链反应的定量分析表明，使用基于趋流性的微流控装置以最佳流速（25 μL/min）选择的精子中没有任何病毒。
我们开发了一种简单，经济有效的微流控装置，该装置在辅助生殖技术的选择过程中有效地洗涤原始精液的同时，模拟了女性生殖道的条件。
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