Division of Animal Science, School of Agriculture and Natural Resources, University of Phayao, Phayao, Thailand.
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiangmai University, Chiangmai, Thailand.
Reprod Domest Anim. 2022 Aug;57(8):928-935. doi: 10.1111/rda.14155. Epub 2022 May 25.
Egg yolk is a common cryoprotectant that can be used as a semen extender to protect the spermatozoa from damage during cryopreservation. Therefore, the aim of this study was to investigate the efficacy of fresh and lyophilized egg yolk, as a Tris-base extender, on the quality of cryopreserved goat semen. Semen from 10 rams of two different breeds (Boer and Saanen) was collected using an artificial vagina. Each ejaculate sample was divided into four equal aliquots, which contained 20% of the fresh egg yolk (a control group), and then 10%, 15%, and 20% of the lyophilized egg yolk as a Tris-base extender. Sperm motility and kinetic parameters were determined using a computer-assisted semen analyser. The results showed that the addition of 20% of the fresh egg yolk in Tris-base extender exhibited significantly higher progressive motility, progressive fast motility, distance curve line, and beat-cross frequency parameters in the post-thaw Boer and Saanen goat sperm when compared with the addition of 10%, 15%, and 20% of the lyophilized egg yolk. The percentage of total motility and immotile parameters in the post-thaw Boer and Saanen goat sperm were not significantly different between the control and 10%, 15% as well as 20% of the lyophilized egg yolk groups. Moreover, the percentage of viability parameter in the Boer and Saanen goat sperm was not significantly different between the control and 10% of the lyophilized egg yolk group but showed significant difference between the control group and 15% and 20% of the lyophilized egg yolk groups. Furthermore, the interaction between the two breeds was significantly different in terms of head activity and straightness parameter. In conclusion, the treatment with 20% of fresh egg yolk in Tris-base extender is superior to the lyophilized egg yolk. However, an addition of 10% of the lyophilized egg yolk in Tris-base extender presented the percentage of total motility and viability parameters showing no difference with 20% of fresh egg yolk. Therefore, 10% of the lyophilized egg yolk in Tris-base extender provided detail of the lyophilized egg yolk protocol in cryopreserved goat semen as an example of an alternative extender to 20% of fresh egg yolk for situations where an animal's origin represents a microbiological risk.
蛋黄是一种常见的抗冻保护剂，可用作精液稀释液，以保护精子在冷冻保存过程中免受损伤。因此，本研究旨在探讨新鲜蛋黄和冻干蛋黄作为 Tris 基础稀释液对冷冻山羊精液质量的影响。使用人工阴道从 10 只不同品种（布尔和萨能）的公羊中采集精液。每个精液样本被分成四个相等的等分，其中含有 20%的新鲜蛋黄（对照组），然后是 10%、15%和 20%的冻干蛋黄作为 Tris 基础稀释液。使用计算机辅助精液分析系统测定精子运动和动力学参数。结果表明，与添加 10%、15%和 20%的冻干蛋黄相比，在 Tris 基础稀释液中添加 20%的新鲜蛋黄可显著提高解冻后布尔和萨能山羊精子的前向运动、快速前向运动、距离曲线线和鞭打交叉频率参数。解冻后布尔和萨能山羊精子的总运动和不动参数百分比在对照组与 10%、15%和 20%的冻干蛋黄组之间无显著差异。此外，在布尔和萨能山羊精子中，活力参数的百分比在对照组与 10%的冻干蛋黄组之间无显著差异，但在对照组与 15%和 20%的冻干蛋黄组之间有显著差异。此外，两个品种之间在头部活动和直线度参数方面的相互作用有显著差异。总之，在 Tris 基础稀释液中添加 20%的新鲜蛋黄优于冻干蛋黄。然而，在 Tris 基础稀释液中添加 10%的冻干蛋黄与添加 20%的新鲜蛋黄相比，总运动和活力参数的百分比无差异。因此，在 Tris 基础稀释液中添加 10%的冻干蛋黄可以作为替代 20%新鲜蛋黄的方法，为冷冻山羊精液提供详细的冻干蛋黄方案，例如在动物来源代表微生物风险的情况下。
