Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Chem Biodivers. 2022 Jun;19(6):e202100753. doi: 10.1002/cbdv.202100753. Epub 2022 May 17.
Aiming at the excellent killing effect of N-phenylmaleimide (N-PMI) on microorganisms, this article used structural simulation analysis, fluorescence analysis, confocal laser scanning microscope and SEM to find that the double bond in N-PMI could interact with the sulfur groups in the membrane protein, changing its conformation, rupturing the plasma membrane of the cell, leaking the contents, and ultimately causing the death of the microorganisms. Therefore, once the double bond participated in the polymerization, N-PMI lost its antimicrobial function. N-PMI could achieve azeotropic copolymerization with MMA through reactive extrusion polymerization. N-PMI with a content of 5 % can be evenly inserted into the PMMA chain segment during the copolymerization reaction, thereby increasing the Tg of pure PMMA by up to 15 °C, which provided the PMMA-co-PMI copolymer with resistance to boiling water sterilization advantageous conditions. In addition, N-PMI with a content of 5 % has little effect on the transparency of PMMA after participating in the copolymerization. Moreover, the trace amount of residual N-PMI made the material have excellent antimicrobial function, and the bacteriostatic zone is extremely small, which provided an excellent guarantee for the safety and durability of the material. As a medical biological material, the PMMA-co-PMI copolymer has a good industrialization application prospects.
针对 N- 苯基马来酰亚胺（N-PMI）对微生物的优异杀灭效果，本文通过结构模拟分析、荧光分析、共聚焦激光扫描显微镜和 SEM 发现，N-PMI 中的双键可以与膜蛋白中的硫基团相互作用，改变其构象，破坏细胞的质膜，使内容物泄漏，最终导致微生物死亡。因此，一旦双键参与聚合，N-PMI 就会失去其抗菌功能。N-PMI 可以通过反应挤出聚合与 MMA 实现共沸共聚。N-PMI 的含量为 5%时，可以在共聚反应中均匀地插入 PMMA 链段中，从而使纯 PMMA 的玻璃化转变温度（Tg）提高 15°C 以上，为 PMMA-co-PMI 共聚物提供了耐沸水灭菌的有利条件。此外，N-PMI 含量为 5%时，参与共聚后对 PMMA 的透明度几乎没有影响。而且，痕量残留的 N-PMI 使材料具有优异的抗菌功能，抑菌圈极小，为材料的安全性和耐用性提供了极好的保障。作为一种医用生物材料，PMMA-co-PMI 共聚物具有良好的工业化应用前景。
