Bu Wen-Han, Asilebieke Ayakuzi, Han Lu-Yang, Xu Yang, Zhou Tao, Chu Jian-Jun
Department of Orthopedics, The Second People's Hospital of Hefei, Hefei Hospital, Affiliated to Anhui Medical University No. 246 of Heping Road, Yaohai District Hefei Anhui 230011 China
The Fifth Clinical Medical School of Anhui Medical University Hefei Anhui 230032 China.
RSC Adv. 2025 Jan 10;15(2):806-813. doi: 10.1039/d4ra06607c. eCollection 2025 Jan 9.
: to address the issue of burst drug release in antibiotic-loaded poly(methyl methacrylate) (PMMA) bone cement (ALBC), this study involved preparation of novel PMMA bone cement and determination of its antibacterial activity, biocompatibility, compressive properties, maximum temperature, and setting time. : a novel acrylic monomer, which contains the 3,4-dichloro-5-hydroxyfuran-2(5)-one (DHF), was synthesized and utilized to develop non-leaching antibacterial PMMA bone cement (NLBC), designated as DHF-methacrylic acid (DHF-MAA) bone cement. In the preparation of this bone cement, DHF-MAA served as a key component of the liquid phase. Its antibacterial activity was determined using a surface antibacterial assay. The biocompatibility of the cement was evaluated through a rabbit-suspended erythrocyte hemolysis test, assessment of the relative proliferation rate of mouse embryonic osteoblast precursor cells (MC3T3-E1) using the CCK-8 method, and an acute toxicity test in mice. The assessment of compressive properties includes both compressive strength and compressive modulus before and after aging. : DHF-MAA bone cement exhibited antibacterial activity, excellent biocompatibility, and acceptable compressive properties; in particular, the 10% DHF-MAA bone cement, achieved 100% antibacterial activity, excellent biocompatibility, and a compressive strength that met the compressive value, as stated in ISO 5833. : in this study, novel antibacterial non-leaching DHF-MAA bone cement was synthesized and evaluated for its antibacterial activity, biocompatibility, and compressive properties. In particular, the 10% DHF-MAA bone cement exhibited excellent antibacterial activity, biocompatibility, and acceptable compressive properties. As such, this cement formulation warrants further characterization with a view to using it to anchor cemented arthroplasties.
为解决载抗生素聚甲基丙烯酸甲酯（PMMA）骨水泥（ALBC）中药物突发释放的问题，本研究涉及新型PMMA骨水泥的制备及其抗菌活性、生物相容性、压缩性能、最高温度和凝固时间的测定。合成了一种含3,4-二氯-5-羟基呋喃-2(5)-酮（DHF）的新型丙烯酸单体，并用于开发非浸出性抗菌PMMA骨水泥（NLBC），即DHF-甲基丙烯酸（DHF-MAA）骨水泥。在制备这种骨水泥时，DHF-MAA作为液相的关键成分。其抗菌活性通过表面抗菌试验测定。通过兔悬浮红细胞溶血试验、使用CCK-8法评估小鼠胚胎成骨细胞前体细胞（MC3T3-E1）的相对增殖率以及小鼠急性毒性试验来评估骨水泥的生物相容性。压缩性能评估包括老化前后的抗压强度和压缩模量。DHF-MAA骨水泥表现出抗菌活性、优异的生物相容性和可接受的压缩性能；特别是10% DHF-MAA骨水泥实现了100%的抗菌活性、优异的生物相容性以及符合ISO 5833规定的压缩强度值。在本研究中，合成了新型抗菌非浸出性DHF-MAA骨水泥，并对其抗菌活性、生物相容性和压缩性能进行了评估。特别是10% DHF-MAA骨水泥表现出优异的抗菌活性、生物相容性和可接受的压缩性能。因此，这种骨水泥配方有待进一步表征，以期用于固定骨水泥型关节置换术。
