Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Animal Laboratory Center, West China Hospital, Sichuan University, China.
Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, 611130, Sichuan, China.
Microb Pathog. 2022 Jun;167:105572. doi: 10.1016/j.micpath.2022.105572. Epub 2022 May 10.
The protective effect of cinnamaldehyde on channel catfish infected by drug-resistant Aeromonas hydrophila CW strain was explored by observing the clinical signs and histopathology, measuring the cumulative mortality, serum biochemical and non-specific immune indicators, and intestinal microbiota in this study. The cumulative survival rate of the cinnamaldehyde within 14 days was significantly higher than that of the challenge group, which was 70% and 20%, respectively. Compared with the challenge group, the activities of lysozyme, superoxide dismutase, and glutathione peroxidase in the treatment group were increased, while there was no significant difference in catalase activity. Compared with the challenge group, the histopathology results showed that the injury of liver, spleen, and kidney was significantly alleviated after cinnamaldehyde treatment. The results of intestinal microbiota showed that the proportion of Proteobacteria in the challenge group was significantly increased, and the proportion of Aeromonas sp. reached 30% based on the analysis of species classification level. The composition of dominant species in the treatment group was similar to the control group. In conclusion, cinnamaldehyde increased the cumulative survival rate of channel catfish infected by A. hydrophila. It could protect channel catfish through improving the non-specific immune function of channel catfish, alleviating the pathological lesions of liver, spleen, kidney, and intestine, and maintaining the relative balance of the intestinal microbiota. Therefore, cinnamaldehyde could be a candidate drug for the treatment of A. hydrophila infection.
本研究通过观察临床症状和组织病理学变化、测定累计死亡率、血清生化和非特异性免疫指标以及肠道微生物群，探讨了肉桂醛对感染耐药嗜水气单胞菌 CW 株的斑点叉尾鮰的保护作用。在 14 天内，肉桂醛组的累计存活率明显高于攻毒组，分别为 70%和 20%。与攻毒组相比，治疗组溶菌酶、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性增加，而过氧化氢酶的活性没有显著差异。与攻毒组相比，组织病理学结果表明，肉桂醛处理后肝脏、脾脏和肾脏的损伤明显减轻。肠道微生物群的结果表明，攻毒组中变形菌门的比例显著增加，基于物种分类水平的分析，气单胞菌属的比例达到 30%。治疗组的优势种组成与对照组相似。综上所述，肉桂醛提高了感染嗜水气单胞菌的斑点叉尾鮰的累计存活率。它可以通过提高斑点叉尾鮰的非特异性免疫功能、减轻肝、脾、肾和肠道的病理损伤以及维持肠道微生物群的相对平衡来保护斑点叉尾鮰。因此，肉桂醛可能是治疗嗜水气单胞菌感染的候选药物。
