Dong Jing, Tong Jian, Li Shengping, Ma Xinwei, Yang Qiuhong, Liu Yongtao, Zhou Shun, Shi Xizhi, Ai Xiaohui
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410125, China.
Biology (Basel). 2025 Apr 27;14(5):483. doi: 10.3390/biology14050483.
Aquatic products play a crucial role in meeting the increasing global demands for high-quality proteins. However, the occurrence of bacterial diseases results in significant economic losses worldwide. () is the pathogen of several fish diseases. Antibiotics were widely used in combating bacterial diseases in aquaculture. The increasing occurrences of antibiotic resistance necessitate the restricted use of antibiotics. Consequently, developing drugs that avoid antibiotic resistance is important for the future of aquaculture. Quorum sensing (QS) is critical for bacterial pathogens in regulating bacterial virulence and is a promising target for developing anti-infective agents. Here, we found that turmeric oil with a MIC of 256 μg/mL could dose-dependently reduce the virulence phenotypes regulated by QS, ranging from 8 to 64 μg/mL, suggesting that sub-inhibitory concentrations of turmeric oil could inhibit bacterial virulence. Further qPCR findings demonstrated that turmeric oil could significantly inhibit the transcription of , , and by a 54-fold, 36-fold, and 56-fold change reduction, respectively. Cell live/dead staining and animal study results showed that turmeric oil could inhibit the pathogenicity of . Fish treated with turmeric oil showed a reduced mortality rate of 60%, whereas all fish in the positive control group died. Moreover, treatment with turmeric oil could alleviate the renal injury. Collectively, the results suggested that targeting bacterial virulence might be a useful approach to combating bacterial infections, and turmeric oil could serve as a potential agent for combating infections.
水产品在满足全球对优质蛋白质日益增长的需求方面发挥着关键作用。然而，细菌性疾病的发生在全球范围内造成了重大经济损失。（）是几种鱼类疾病的病原体。抗生素曾被广泛用于对抗水产养殖中的细菌性疾病。抗生素耐药性的日益增加使得抗生素的使用受到限制。因此，开发避免抗生素耐药性的药物对水产养殖的未来至关重要。群体感应（QS）对于细菌病原体调节细菌毒力至关重要，并且是开发抗感染药物的一个有前景的靶点。在此，我们发现最低抑菌浓度为256μg/mL的姜黄油能够以剂量依赖的方式降低由QS调节的毒力表型，浓度范围为8至64μg/mL，这表明亚抑菌浓度的姜黄油能够抑制细菌毒力。进一步的qPCR结果表明，姜黄油能够分别以54倍、36倍和56倍的变化降低幅度显著抑制（相关基因）的转录。细胞死活染色和动物研究结果表明，姜黄油能够抑制（病原体）的致病性。用姜黄油处理的鱼死亡率降低了60%，而阳性对照组的所有鱼都死亡了。此外，用姜黄油处理可以减轻肾脏损伤。总体而言，结果表明针对细菌毒力可能是对抗细菌感染的一种有效方法，并且姜黄油可以作为对抗（相关病原体）感染的一种潜在药物。
