蓖麻毒素A链在大肠杆菌中的表达。
Expression of ricin A chain in Escherichia coli.
作者信息
O'Hare M, Roberts L M, Thorpe P E, Watson G J, Prior B, Lord J M
出版信息
FEBS Lett. 1987 May 25;216(1):73-8. doi: 10.1016/0014-5793(87)80759-7.
PMID:3556218
Abstract
DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.
摘要
编码蓖麻毒素A链的DNA源自前蓖麻毒素cDNA，并被连接到表达载体pDS5/3中。转录由与大肠杆菌乳糖操纵子融合的噬菌体启动子PN25控制。诱导后，用重组质粒转化的大肠杆菌71.18细胞表达出具有溶解性且具有完整生物活性的蓖麻毒素A链。
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