蓖麻毒素B链在大肠杆菌中的表达。
Expression of ricin B chain in Escherichia coli.
作者信息
Hussain K, Bowler C, Roberts L M, Lord J M
机构信息
Department of Biological Sciences, University of Warwick, Coventry, England.
出版信息
FEBS Lett. 1989 Feb 27;244(2):383-7. doi: 10.1016/0014-5793(89)80568-x.
PMID:2646152
Abstract
DNA encoding ricin B chain was fused to that encoding the E. coli OmpA signal peptide using the expression secretion vector pIN-111-ompA. When induced, E. coli cells transformed with the recombinant plasmid express ricin B chain. The recombinant product accumulates in the periplasmic space in a soluble, biologically active form.
摘要
利用表达分泌载体pIN-111-ompA，将编码蓖麻毒素B链的DNA与编码大肠杆菌OmpA信号肽的DNA融合。当诱导时，用重组质粒转化的大肠杆菌细胞表达蓖麻毒素B链。重组产物以可溶的、具有生物活性的形式积累在周质空间中。
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