School of Chemistry, University of New South Wales, Sydney, New South Wales, 2052, Australia.
School of Chemical Engineering, University of New South Wales, Sydney, New South Wales, 2052, Australia.
Trends Biotechnol. 2022 Nov;40(11):1374-1392. doi: 10.1016/j.tibtech.2022.04.004. Epub 2022 May 11.
Owing to recent advances in mass spectrometry (MS), tens to hundreds of proteins, lipids, and small molecules can be measured in single cells. The ability to characterize the molecular heterogeneity of individual cells is necessary to define the full assortment of cell subtypes and identify their function. We review single-cell MS including high-throughput, targeted, mass cytometry-based approaches and antibody-free methods for broad profiling of the proteome and metabolome of single cells. The advantages and disadvantages of different methods are discussed, as well as the challenges and opportunities for further improvements in single-cell MS. These methods is being used in biomedicine in several applications including revealing tumor heterogeneity and high-content drug screening.
由于质谱（MS）技术的最新进展，现在可以在单个细胞中测量数十到数百种蛋白质、脂质和小分子。对单个细胞的分子异质性进行特征描述是定义完整的细胞亚型并确定其功能所必需的。我们综述了单细胞 MS，包括高通量、靶向、基于质谱细胞术的方法和抗体自由的方法，用于对单细胞的蛋白质组和代谢组进行广泛的分析。讨论了不同方法的优缺点，以及单细胞 MS 进一步改进的挑战和机遇。这些方法正在包括揭示肿瘤异质性和高通量药物筛选在内的多个应用于生物医学领域。
