University of Tuebingen, CMFI Cluster of Excellence, Interfaculty Institute of Microbiology and Infection Medicine, Tuebingen, Germany.
University of Tuebingen, Center for Plant Molecular Biology, Tuebingen, Germany.
Proteomics. 2024 Jun;24(12-13):e2200533. doi: 10.1002/pmic.202200533. Epub 2023 Nov 21.
With the emergence of next-generation nucleotide sequencing and mass spectrometry-based proteomics and metabolomics tools, we have comprehensive and scalable methods to analyze the genes, transcripts, proteins, and metabolites of a multitude of biological systems. Despite the fascinating new molecular insights at the genome, transcriptome, proteome and metabolome scale, we are still far from fully understanding cellular organization, cell cycles and biology at the molecular level. Significant advances in sensitivity and depth for both sequencing as well as mass spectrometry-based methods allow the analysis at the single cell and single molecule level. At the same time, new tools are emerging that enable the investigation of molecular interactions throughout the central dogma of molecular biology. In this review, we provide an overview of established and recently developed mass spectrometry-based tools to probe metabolite-protein interactions-from individual interaction pairs to interactions at the proteome-metabolome scale.
随着新一代核苷酸测序和基于质谱的蛋白质组学和代谢组学工具的出现，我们有了全面和可扩展的方法来分析众多生物系统的基因、转录本、蛋白质和代谢物。尽管在基因组、转录组、蛋白质组和代谢组水平上有了令人着迷的新分子见解，但我们仍然远远没有完全理解细胞的组织、细胞周期和分子水平上的生物学。测序和基于质谱的方法在灵敏度和深度方面都取得了显著的进展，使得单细胞和单分子水平的分析成为可能。与此同时，新的工具也在不断涌现，使我们能够在整个分子生物学中心法则中研究分子相互作用。在这篇综述中，我们提供了一个基于质谱的工具的概述，用于探测代谢物-蛋白质相互作用，从单个相互作用对到蛋白质组-代谢组水平的相互作用。
