State Key Laboratory of Natural Medicines, Research Center of Biostatistics and Computational Pharmacy, China Pharmaceutical University, Nanjing, 210000, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Nutr J. 2022 May 13;21(1):29. doi: 10.1186/s12937-022-00786-w.
Clarifying the association between 5-methyltetrahydrofolate and homocysteine and the effect pattern of methylene tetrahydrofolate reductase (MTHFR C677T) may contribute to the management of homocysteine and may serve as a significant reference for a randomized controlled trial of 5-methyltetrahydrofolate intervention. This study aimed to reveal the association between these two biochemical indices.
Study population was drawn from the baseline data of the China Stroke Primary Prevention Trial (CSPPT), including 2328 hypertensive participants. 5-methyltetrahydrofolate and homocysteine were determined by stable-isotope dilution liquid chromatography-tandem mass spectrometry and automatic clinical analyzers, respectively. MTHFR C677T polymorphisms were detected using TaqMan assay. Multiple linear regression was performed to evaluate the association between serum 5-methyltetrahydrofolate and homocysteine.
There was a significant inverse association between 5-methyltetrahydrofolate and homocysteine when 5-methyltetrahydrofolate was ≤ 10 ng/mL, and this association was modified by MTHFR C677T (per 1-ng/mL increment; All: β = - 0.50, P < 0.001; CC: β = - 0.14, P = 0.087; CT: β = - 0.20, P = 0.011; TT: β = - 1.19, P < 0.001). Moreover, the decline in trend in genotype TT participants was stronger than in genotype CC participants (P for difference < 0.001) and genotype CT participants (P for difference < 0.001), while there was no significant difference between genotype CC and genotype CT participants (P for difference = 0.757).
Our data showed a non-linear association between serum homocysteine and 5-methyltetrahydrofolate among Chinese hypertensive adults, however, it could be inversely linearly fitted when serum 5-methyltetrahydrofolate was ≤ 10 ng/mL, and this association was modified by MTHFR C677T.
阐明 5-甲基四氢叶酸（5-MTHF）与同型半胱氨酸之间的关联以及亚甲基四氢叶酸还原酶（MTHFR C677T）的作用模式可能有助于同型半胱氨酸的管理，并为 5-MTHF 干预的随机对照试验提供重要参考。本研究旨在揭示这两个生化指标之间的关联。
本研究的研究人群来自中国脑卒中一级预防试验（CSPPT）的基线数据，包括 2328 名高血压参与者。5-MTHF 和同型半胱氨酸分别采用稳定同位素稀释液相色谱-串联质谱法和自动临床分析仪进行测定。MTHFR C677T 多态性采用 TaqMan 法检测。采用多元线性回归评估血清 5-MTHF 与同型半胱氨酸之间的关系。
当血清 5-MTHF ≤ 10ng/mL 时，5-MTHF 与同型半胱氨酸之间存在显著负相关，这种关联受 MTHFR C677T 影响（每增加 1ng/mL，所有：β = -0.50，P < 0.001；CC：β = -0.14，P = 0.087；CT：β = -0.20，P = 0.011；TT：β = -1.19，P < 0.001）。此外，TT 基因型参与者的趋势下降幅度强于 CC 基因型参与者（P 差值 < 0.001）和 CT 基因型参与者（P 差值 < 0.001），而 CC 基因型和 CT 基因型参与者之间无显著差异（P 差值 = 0.757）。
本研究数据显示，中国高血压成年人血清同型半胱氨酸与 5-MTHF 之间存在非线性关系，但当血清 5-MTHF ≤ 10ng/mL 时，这种关系可呈负线性拟合，且该关联受 MTHFR C677T 影响。
