State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A&F University, Xianyang 712100, China.
Nanyang Academy of Agricultural Sciences, Nanyang 473000, China.
Int J Mol Sci. 2022 Apr 20;23(9):4519. doi: 10.3390/ijms23094519.
Drought stress is an important factor that severely affects crop yield and quality. Autophagy has a crucial role in the responses to abiotic stresses. In this study, we explore in response to drought stress. Expression of the gene was strongly induced by NaCl, PEG, and abscisic acid treatments. The TaNBR1 protein is localized in the Golgi apparatus and autophagosome. Transgenic plants overexpressing exhibited reduced drought tolerance. When subjected to drought stress, compared to the wild-type (WT) lines, the transgenic overexpressing plants had a lower seed germination rate, relative water content, proline content, and reduced accumulation of antioxidant enzymes, i.e., superoxide dismutase, peroxidase, and catalase, as well as higher chlorophyll losses, malondialdehyde contents, and water loss. The transgenic plants overexpressing produced much shorter roots in response to mannitol stress, in comparison to the WT plants, and they exhibited greater sensitivity to abscisic acid treatment. The expression levels of the genes related to stress in the transgenic plants were affected in response to drought stress. Our results indicate that negatively regulates drought stress responses by affecting the expression of stress-related genes in .
干旱胁迫是严重影响作物产量和品质的重要因素。自噬在非生物胁迫响应中起关键作用。本研究探讨了 TaNBR1 基因在小麦中的功能。TaNBR1 基因受 NaCl、PEG 和脱落酸处理强烈诱导表达。TaNBR1 蛋白定位于高尔基体和自噬体。过表达 TaNBR1 的转基因植株表现出降低的耐旱性。与野生型（WT）系相比，在干旱胁迫下，过表达 TaNBR1 的转基因植株的种子发芽率、相对水含量、脯氨酸含量较低，抗氧化酶（如超氧化物歧化酶、过氧化物酶和过氧化氢酶）的积累减少，以及叶绿素损失、丙二醛含量和水分损失较高。与 WT 植株相比，过表达 TaNBR1 的转基因植株在甘露醇胁迫下产生的根明显更短，对脱落酸处理更敏感。干旱胁迫下，转基因植株中与胁迫相关的基因表达水平受到影响。我们的结果表明，TaNBR1 通过影响 中的胁迫相关基因的表达来负调控干旱胁迫响应。
