Molecular Population Genetics and Breeding Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore.
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Int J Mol Sci. 2022 Apr 22;23(9):4659. doi: 10.3390/ijms23094659.
Oil palm is the most productive oil producing plant. Salt stress leads to growth damage and a decrease in yield of oil palm. However, the physiological responses of oil palm to salt stress and their underlying mechanisms are not clear. RNA-Seq was conducted on control and leaf samples from young palms challenged under three levels of salts (100, 250, and 500 mM NaCl) for 14 days. All three levels of salt stress activated EgSPCH expression and increased stomatal density of oil palm. Around 41% of differential expressed genes (DEGs) were putative EgSPCH binding target and were involved in multiple bioprocesses related to salt response. Overexpression of EgSPCH in Arabidopsis increased the stomatal production and lowered the salt tolerance. These data indicate that, in oil palm, salt activates EgSPCH to generate more stomata in response to salt stress, which differs from herbaceous plants. Our results might mirror the difference of salt-induced stomatal development between ligneous and herbaceous crops.
油棕是产油量最高的油料作物。盐胁迫会导致油棕生长受损和产量下降。然而，油棕对盐胁迫的生理响应及其潜在机制尚不清楚。对对照和在三种盐浓度（100、250 和 500mM NaCl）下处理 14 天的幼龄油棕叶片样本进行了 RNA-Seq 分析。所有三种盐胁迫水平都激活了 EgSPCH 的表达并增加了油棕的气孔密度。约 41%的差异表达基因（DEGs）是推定的 EgSPCH 结合靶标，涉及与盐响应相关的多个生物过程。在拟南芥中过表达 EgSPCH 会增加气孔的产生并降低盐耐受性。这些数据表明，在油棕中，盐激活 EgSPCH 以响应盐胁迫产生更多的气孔，这与草本植物不同。我们的结果可能反映了木本和草本作物盐诱导气孔发育的差异。
