Institute of Agrophysics of Polish Academy of Sciences, 20-290 Lublin, Poland.
Int J Mol Sci. 2022 May 6;23(9):5182. doi: 10.3390/ijms23095182.
Plants grow in a variable environment in regard to soil water and light driving photochemical reactions. Light energy exceeding plant capability to use it for photochemical reactions must be dissipated by processes of non-photochemical quenching (NPQ). The aim of the study was to evaluate the impact of various components of NPQ on the response of to fluctuating light and water availability. A laboratory experiment with wild type (WT) and mutants npq1 and npq4 grown under optimum or reduced water availability was conducted. Dark-adapted plants were illuminated with fluctuating light (FL) of two intensities (55 and 530 μmol m s) with each of the phases lasting for 20 s. The impact of water availability on the role of zeaxanthin and PsbS protein in NPQ induced at FL was analysed. The water deficit affected the dynamics of NPQ induced by FL. The lack of zeaxanthin or PsbS reduced plant capability to cope with FL. The synergy of both of these components was enhanced in regard to the amplitude of NPQ in the drought conditions. PsbS was shown as a component of primary importance in suiting plant response to FL under optimum and reduced water availability.
植物在土壤水分和光照变化的环境中生长，这些变化会驱动光化学反应。超过植物光化学反应能力的光能必须通过非光化学猝灭（NPQ）过程耗散。本研究旨在评估 NPQ 的各个组成部分对植物响应波动光照和水分供应的影响。在最佳水分供应或减少水分供应的条件下，对野生型（WT）和 npq1 和 npq4 突变体进行了实验室实验。暗适应的植物用两种强度（55 和 530 μmol m s）的波动光（FL）进行照射，每个阶段持续 20 s。分析了水分供应对 FL 诱导的玉米黄质和 PsbS 蛋白在 NPQ 中作用的影响。水分亏缺影响了 FL 诱导的 NPQ 的动力学。缺乏玉米黄质或 PsbS 会降低植物应对 FL 的能力。在干旱条件下，这两个组成部分在 NPQ 的幅度上表现出协同作用。PsbS 被证明是在最佳和减少水分供应条件下适应植物对 FL 响应的一个重要组成部分。
