Instrument Sharing Platform of School of Life Sciences, East China Normal University, Shanghai 200241, China.
Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
Int J Mol Sci. 2022 May 6;23(9):5194. doi: 10.3390/ijms23095194.
Trehalose can improve the tolerance of plants to various types of environmental stress. Nonetheless, information respecting the molecular networks of wheat seedlings to exogenous trehalose under heat stress is limited. Here, two wheat varieties pretreated with exogenous trehalose were selected to explore the molecular mechanism by which trehalose improves the heat tolerance of wheat ( L.). The results indicated that exogenous trehalose improved the physiological state of wheat seedlings under heat stress. Through RNA sequencing and metabolomics analysis, the genes and metabolites specifically expressed in trehalose pretreatment were identified. After heat stress, there were 18,352 differentially expressed genes (DEGs) in the control and trehalose-treated (H_vs_TreH) groups of Yangmai 18 and 9045 DEGs in Yannong 19. Functional annotation and enrichment analyses showed that the DEGs in the two wheat varieties were mainly involved in carbohydrate metabolism and biosynthesis of secondary metabolites. Through a liquid chromatography-mass spectrometry platform, 183 differential metabolites in H_vs_TreH groups of Yangmai 18 and 77 differential metabolites in Yannong 19 were identified. Compared with the control group, many protective metabolites, such as amino acids, purines, phenylpropanoids and flavonoids, showed significant differences under heat stress. The results indicated that exogenous trehalose protected the wheat biomembrane system, enhanced carbohydrate metabolism and signal transduction, strengthened the activity of the tricarboxylic acid cycle (TCA cycle), regulated purine metabolism, gene expression and metabolite accumulation in the phenylpropanoid biosynthesis and flavonoid biosynthesis pathways, thus improving the heat tolerance of wheat.
海藻糖可以提高植物对各种环境胁迫的耐受性。然而，关于外源海藻糖在热胁迫下小麦幼苗分子网络的信息有限。在这里，选择了两种用外源海藻糖预处理的小麦品种，以探索海藻糖提高小麦（L.）耐热性的分子机制。结果表明，外源海藻糖改善了热胁迫下小麦幼苗的生理状态。通过 RNA 测序和代谢组学分析，鉴定了海藻糖预处理中特异性表达的基因和代谢物。在热胁迫后，扬麦 18 的对照和海藻糖处理（H_vs_TreH）组中有 18352 个差异表达基因（DEGs），而烟农 19 中有 9045 个 DEGs。功能注释和富集分析表明，两个小麦品种的 DEGs 主要参与碳水化合物代谢和次生代谢物的生物合成。通过液相色谱-质谱平台，鉴定了扬麦 18 的 H_vs_TreH 组中有 183 个差异代谢物，而烟农 19 中有 77 个差异代谢物。与对照组相比，许多保护代谢物，如氨基酸、嘌呤、苯丙烷和类黄酮，在热胁迫下表现出明显的差异。结果表明，外源海藻糖保护了小麦生物膜系统，增强了碳水化合物代谢和信号转导，增强了三羧酸循环（TCA 循环）的活性，调节了嘌呤代谢、苯丙烷生物合成和类黄酮生物合成途径中的基因表达和代谢物积累，从而提高了小麦的耐热性。
