PG School, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
ICAR-National Institute for Plant Biotechnology, New Delhi, 110012, India.
Sci Rep. 2022 Sep 1;12(1):14831. doi: 10.1038/s41598-022-18625-7.
Wheat, one of the most widely consumed staple food crops globally, is relatively vulnerable to high temperature-induced heat stress. It is therefore essential to gain more insight into the comprehensive mechanism of thermotolerance of wheat in order to safeguard its production. In view of this, we analysed heat stress responsive transcriptome data of wheat to determine its gene expression level under heat stress. A total of 7990 DEGs, including 4483 up-regulated and 3507 down regulated genes were identified. Gene Ontology (GO) analysis categorized 3910 DEGs into different ontology families. 146 pathways involving 814 DEGs were enriched during KEGG analysis. Metabolic pathways and biosynthesis of secondary metabolites were the major pathways enriched. MYB (myeloblastosis) transcription factors (TFs) and many other TFs as bHLH, WRKY, NAC, ERF, were determined to be quite abundant in the DEGs. Since various reports indicate that these TFs play important role in plants abiotic stress, it is an indication that our DEGs are functional in heat stress tolerance. Verification of few selected DEGs using RT-qPCR produced expression levels similar to the transcriptome data. This indicates that the transcriptome data is reliable. These results could be helpful in enhancing our understanding of the mechanism underlying thermotolerance in wheat.
小麦是全球广泛食用的主要粮食作物之一，相对容易受到高温引起的热应激的影响。因此，深入了解小麦的耐热综合机制对于保障其生产至关重要。有鉴于此，我们分析了小麦热应激响应转录组数据，以确定其在热应激下的基因表达水平。共鉴定出 7990 个差异表达基因，其中包括 4483 个上调基因和 3507 个下调基因。GO 分析将 3910 个 DEGs 分为不同的本体家族。KEGG 分析中富集了 146 条涉及 814 个 DEGs 的途径。代谢途径和次生代谢物的生物合成是主要的富集途径。MYB（髓样细胞瘤）转录因子（TFs）和许多其他 TFs，如 bHLH、WRKY、NAC、ERF，被确定在 DEGs 中相当丰富。由于各种报道表明这些 TFs 在植物非生物胁迫中发挥重要作用，这表明我们的 DEGs 在耐热性中具有功能。使用 RT-qPCR 对少数选定的 DEGs 进行验证，得到的表达水平与转录组数据相似。这表明转录组数据是可靠的。这些结果有助于我们加深对小麦耐热机制的理解。
