Yi Zhengkai, Xie Jing
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China.
Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation, Shanghai 201306, China.
Foods. 2022 Apr 27;11(9):1261. doi: 10.3390/foods11091261.
can cause the spoilage of seafood and shorten its shelf life. In this study, both strains of (YZ08 and YZ-J) isolated from spoiled bigeye tuna were subjected to in-depth phenotypic and genotypic characterization to better understand their roles in seafood spoilage. The complete genome sequences of strains YZ08 and YZ-J were reported. Unique genes of the two strains were identified by pan-genomic analysis. In vitro experiments revealed that YZ08 and YZ-J could adapt to various environmental stresses, including cold-shock temperature, pH, NaCl, and nutrient stresses. YZ08 was better at adapting to NaCl stress, and its genome possessed more NaCl stress-related genes compared with the YZ-J strain. YZ-J was a higher biofilm and exopolysaccharide producer than YZ08 at 4 and 30 °C, while YZ08 showed greater motility and enhanced capacity for biogenic amine metabolism, trimethylamine metabolism, and sulfur metabolism compared with YZ-J at both temperatures. That YZ08 produced low biofilm and exopolysaccharide contents and displayed high motility may be associated with the presence of more a greater number of genes encoding chemotaxis-related proteins (X) and low expression of the A operon. This study provided novel molecular targets for the development of new antiseptic antisepsis strategies.
会导致海鲜变质并缩短其保质期。在本研究中，从变质的大眼金枪鱼中分离出的两株菌（YZ08和YZ-J）进行了深入的表型和基因型特征分析，以更好地了解它们在海鲜变质中的作用。报道了YZ08和YZ-J菌株的全基因组序列。通过泛基因组分析鉴定了这两株菌的独特基因。体外实验表明，YZ08和YZ-J能够适应各种环境压力，包括冷休克温度、pH值、NaCl和营养压力。YZ08更能适应NaCl压力，与YZ-J菌株相比，其基因组拥有更多与NaCl压力相关的基因。在4℃和30℃时，YZ-J比YZ08产生更多的生物膜和胞外多糖，而在这两个温度下，YZ08与YZ-J相比表现出更大的运动性以及在生物胺代谢、三甲胺代谢和硫代谢方面增强的能力。YZ08产生低含量的生物膜和胞外多糖并表现出高运动性，这可能与存在更多编码趋化相关蛋白（X）的基因以及A操纵子的低表达有关。本研究为开发新的防腐策略提供了新的分子靶点。
