College of Food Science & Technology, Shanghai Ocean University, Shanghai, 201306, China.
Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Shanghai Ocean University, Shanghai, 201306, China.
J Agric Food Chem. 2023 Jun 21;71(24):9558-9568. doi: 10.1021/acs.jafc.3c02113. Epub 2023 Jun 12.
is a typical spoiler that is commonly found in seafood and has high spoilage potential. However, the spoilage mechanism against at the gene and metabolism levels has not been well elucidated. This work determined the spoilage targets on XY07 from spoiled bigeye tuna by genome sequencing, metabolomics, and Fourier transform infrared (FTIR) analysis. XY07 contained some genes on spoilage regulating of genes, genes, genes and gene involved in sulfur metabolism, histidine metabolism, arginine and proline degradation, and biofilm formation at the genome level, respectively. Some spoilage genes like genes were identified. In addition, ABC transporters, arginine and proline metabolism; beta-alanine metabolism; glycine, serine, and threonine metabolism; histidine metabolism; sulfur metabolism; and lipid metabolism were identified as important pathways related to aquatic food during spoilage, which indicated the functions of amino acid degradation in XY 07 by metabolomics analysis. The metabolites of l-ornithine, 5-aminopentanoate, and 4-aminobutyraldehyde could be further metabolized to spermidine and spermine, producing a spoilage odor, and were involved in arginine and proline metabolism serving as key spoilage regulating metabolisms. Therefore, XY07 was applied to genomics, metabolomics analysis, and FTIR to provide comprehensive insight into the investigation of spoilage targets.
是一种常见的海鲜中存在的典型腐败菌，具有很高的腐败潜力。然而，其在基因和代谢水平上的腐败机制尚未得到很好的阐明。本研究通过基因组测序、代谢组学和傅里叶变换红外（FTIR）分析，确定了从腐败大眼金枪鱼中分离得到的 XY07 的腐败靶点。XY07 在基因组水平上分别含有一些与基因、基因、基因和基因参与的硫代谢、组氨酸代谢、精氨酸和脯氨酸降解以及生物膜形成有关的基因。鉴定出了一些与腐败有关的基因，如基因。此外，ABC 转运蛋白、精氨酸和脯氨酸代谢；β-丙氨酸代谢；甘氨酸、丝氨酸和苏氨酸代谢；组氨酸代谢；硫代谢；和脂质代谢被确定为与水产食品腐败有关的重要途径，这表明代谢组学分析中 XY07 中氨基酸降解的功能。l-鸟氨酸、5-氨基戊酸和 4-氨基丁醛的代谢物可以进一步代谢为腐胺和精胺，产生腐败气味，并参与精氨酸和脯氨酸代谢，作为关键的腐败调节代谢物。因此，XY07 被应用于基因组学、代谢组学分析和 FTIR，为腐败靶点的调查提供了全面的见解。
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