Quattrochi J J, Madison R, Sidman R L, Kljavin I
Exp Neurol. 1987 Apr;96(1):219-24. doi: 10.1016/0014-4886(87)90184-1.
A new retrograde tracer, rhodamine latex microspheres, permits labeled neurons to be visualized with fluorescence light microscopy. However, their use has been limited to the light microscope. We now have developed colloidal gold fluorescent microspheres which identify retrogradely labeled neurons first by fluorescence microscopy and then by electron microscopy. This new fluorescent/EM tracer will find widespread use in the field of neuroscience to elucidate the ultrastructural integrity of neuronal networks.
一种新型逆行示踪剂——罗丹明乳胶微球，可通过荧光显微镜观察到标记的神经元。然而，其应用仅限于光学显微镜。我们现已开发出胶体金荧光微球，它首先通过荧光显微镜识别逆行标记的神经元，然后通过电子显微镜进行观察。这种新型荧光/电子显微镜示踪剂将在神经科学领域得到广泛应用，以阐明神经网络的超微结构完整性。
