Davey Matthew G, Feeney Gerard, Annuk Heidi, Paganga Maxwell, Holian Emma, Lowery Aoife J, Kerin Michael J, Miller Nicola
Department of Surgery, Lambe Institute for Translational Research, National University of Ireland, H91 YR71 Galway, Ireland.
School of Mathematical and Statistical Sciences, National University of Ireland, H91 H3CY Galway, Ireland.
Cancers (Basel). 2022 Apr 23;14(9):2109. doi: 10.3390/cancers14092109.
Background: Approximately one-third of colorectal cancer (CRC) patients will suffer recurrence. MiRNAs are small non-coding RNAs that play important roles in gene expression. We aimed to correlate miRNA expression with aggressive clinicopathological characteristics and survival outcomes in CRC. Methods: Tumour samples were extracted from 74 CRC patients. MiRNAs were quantified using real-time reverse transcriptase polymerase chain reaction. Descriptive statistics and Cox regression analyses were performed to correlate miRNA targets with clinicopathological and outcome data. Results: Aberrant miR-21 and miR-135b expression correlate with increased nodal stage (p = 0.039, p = 0.022). Using univariable Cox regression analyses, reduced miR-135b (β-coefficient −1.126, hazard ratio 0.324, standard error (SE) 0.4698, p = 0.017) and increased miR-195 (β-coefficient 1.442, hazard ratio 4.229, SE 0.446, p = 0.001) predicted time to disease recurrence. Survival regression trees analysis illustrated a relative cut-off of ≤0.488 for miR-195 and a relative cut-off of >−0.218 for miR-135b; both were associated with improved disease recurrence (p < 0.001, p = 0.015). Using multivariable analysis with all targets as predictors, miR-195 (β-coefficient 3.187, SE 1.419, p = 0.025) was the sole significant independent predictor of recurrence. Conclusion: MiR-195 has strong value in predicting time to recurrence in CRC patients. Additionally, miR-21 and miR-135b predict the degree nodal burden. Future studies may include these findings to personalize therapeutic and surgical decision making.
约三分之一的结直肠癌（CRC）患者会复发。微小RNA（miRNA）是在基因表达中起重要作用的小非编码RNA。我们旨在将miRNA表达与CRC侵袭性临床病理特征及生存结果相关联。方法：从74例CRC患者中提取肿瘤样本。使用实时逆转录聚合酶链反应对miRNA进行定量。进行描述性统计和Cox回归分析，以将miRNA靶点与临床病理及结果数据相关联。结果：miR-21和miR-135b异常表达与淋巴结分期增加相关（p = 0.039，p = 0.022）。使用单变量Cox回归分析，miR-135b降低（β系数−1.126，风险比0.324，标准误（SE）0.4698，p = 0.017）和miR-195增加（β系数1.442，风险比4.229，SE 0.446，p = 0.001）可预测疾病复发时间。生存回归树分析表明，miR-195的相对临界值≤0.488，miR-135b的相对临界值>−0.218；两者均与疾病复发改善相关（p < 0.001，p = 0.015）。以所有靶点作为预测因子进行多变量分析，miR-195（β系数3.187，SE 1.419，p = 0.025）是复发的唯一显著独立预测因子。结论：miR-195在预测CRC患者复发时间方面具有重要价值。此外，miR-21和miR-135b可预测淋巴结负荷程度。未来研究可纳入这些发现以实现治疗和手术决策的个性化。
