Vera Nelson, Gutiérrez-Gómez Constanza, Williams Pamela, Allende Rodrigo, Fuentealba Cecilia, Ávila-Stagno Jorge
Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Campus Chillán, Chillán 3812120, Chile.
Departamento de Producción Animal, Facultad de Agronomía, Universidad de Concepción, Campus Chillán, Chillán 3812120, Chile.
Animals (Basel). 2022 Apr 21;12(9):1080. doi: 10.3390/ani12091080.
The aim of this study was to compare the effects of a pine (Pinus radiata D. Don) bark extract (PBE) with a quebracho (Schinopsis balansae Engl.) extract (QTE) on methane (CH4) production and in vitro rumen fermentation parameters. A forage diet supplemented with PBE or QTE (0, 2 and 4% dry matter (DM) basis) was incubated for 24 h to determine in vitro DM disappearance (IVDMD), CH4, volatile fatty acids (VFA), and ammonia nitrogen (NH3-N) production. Differences were analyzed using Tukey’s test, orthogonal contrasts, hierarchical clustering heatmap (HCH), and principal component analysis (PCA). Both extracts (4% DM) decreased butyrate (Bu; p = 0.001), CH4 (p = 0.005), total VFA (p < 0.001), and NH3-N (p = 0.006) production and increased acetate (Ac; p = 0.003) without affecting the partitioning factor (p = 0.095). Propionate (Pr; p = 0.016) was increased, whereas IVDMD (p = 0.041) was decreased with QTE (4% DM). The inclusion of QTE (2% DM) decreased CH4 production (p = 0.005) and the (Ac + Bu)/Pr ratio (p = 0.003), whereas PBE (2% DM) decreased the NH3-N (p = 0.006) and total VFA production (p < 0.001). The HCH and PCA indicate a negative correlation (r = −0.93; p < 0.001) between CH4 production and tannins. In conclusion, PBE shares many of the effects generated by QTE on ruminal fermentation, although the magnitude of these effects depends on concentration. The PBE could be used as an additive in ruminant diets to reduce CH4 and NH3-N production without reducing IVDMD or increasing propionate, but further in vivo studies are required to clarify its effects on animal production.
本研究的目的是比较辐射松（Pinus radiata D. Don）树皮提取物（PBE）和白坚木（Schinopsis balansae Engl.）提取物（QTE）对甲烷（CH4）产生及体外瘤胃发酵参数的影响。将添加了PBE或QTE（基于干物质（DM）的0%、2%和4%）的草料日粮培养24小时，以测定体外干物质消失率（IVDMD）、CH4、挥发性脂肪酸（VFA）和氨氮（NH3-N）的产生量。使用Tukey检验、正交对比、层次聚类热图（HCH）和主成分分析（PCA）对差异进行分析。两种提取物（4% DM）均降低了丁酸（Bu；p = 0.001）、CH4（p = 0.005）、总VFA（p < 0.001）和NH3-N（p = 0.006）的产生量，并增加了乙酸（Ac；p = 0.003），而不影响分配系数（p = 0.095）。QTE（4% DM）使丙酸（Pr；p = 0.016）增加，而IVDMD（p = 0.041）降低。添加QTE（2% DM）降低了CH4产生量（p = 0.005）和（Ac + Bu）/Pr比值（p = 0.003），而PBE（2% DM）降低了NH3-N（p = 0.006）和总VFA产生量（p < 0.001）。HCH和PCA表明CH4产生量与单宁之间呈负相关（r = -0.93；p < 0.001）。总之，PBE与QTE对瘤胃发酵产生的许多影响相同，尽管这些影响的程度取决于浓度。PBE可作为反刍动物日粮中的添加剂，以减少CH4和NH3-N的产生，而不降低IVDMD或增加丙酸，但需要进一步的体内研究来阐明其对动物生产的影响。
