Brandan E, Inestrosa N C
FEBS Lett. 1987 Mar 9;213(1):159-63. doi: 10.1016/0014-5793(87)81483-7.
We have previously communicated that heparin released asymmetric acetylcholinesterase (AChE) from cholinergic synapses. Here we report studies showing that heparin, besides releasing asymmetric AChE from the skeletal muscle extracellular matrix (ECM), specifically solubilizes a dermatan sulfate proteoglycan (DSPG) which accounts for more than 95% of the 35S-released material. The co-solubilization of AChE and the proteoglycan opens up the possibility that both macromolecules could be involved in the formation of the soluble AChE complex observed after incubation of muscle homogenate with heparin. Our results suggest a possible association between asymmetric AChE and DSPG at the muscle ECM, moreover this work is the first report of the existence of DSPG at the skeletal muscle cell surface.
我们之前曾报道过，肝素可从胆碱能突触释放不对称乙酰胆碱酯酶（AChE）。在此我们报告的研究表明，肝素除了从骨骼肌细胞外基质（ECM）释放不对称AChE外，还能特异性地溶解一种硫酸皮肤素蛋白聚糖（DSPG），该蛋白聚糖占35S释放物质的95%以上。AChE与蛋白聚糖的共同溶解表明，这两种大分子可能都参与了肌肉匀浆与肝素孵育后观察到的可溶性AChE复合物的形成。我们的结果表明，在肌肉ECM中，不对称AChE与DSPG之间可能存在关联，此外，这项工作首次报道了骨骼肌细胞表面存在DSPG。
