State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China.
Nutrients. 2022 Apr 26;14(9):1808. doi: 10.3390/nu14091808.
Harboring various proteins, lipids, and RNAs, the extracellular vesicles (EVs) in milk exert vital tissue-specific immune-protective functions in neonates via these bioactive cargos. This study aims to explore the anti-inflammatory effects of bovine milk-derived EVs on a dextran sulfate sodium (DSS)-induced colitis model and to determine the underlying molecular mechanisms. Sixty C57BL/6 mice were divided into the NC group (normal control), DSS group (DSS + PBS), DSS + LOW group (DSS + 1.5 × 10 p/g EVs), DSS + MID group (DSS + 1.5 × 10 p/g EVs), and DSS + HIG group (DSS + 1.0 × 10 p/g EVs). Histopathological sections, the gut microbiota, and intestinal tissue RNA-Seq were used to comprehensively evaluate the beneficial functions in mitigating colitis. The morphology exhibited that the milk-derived EVs contributed to the integrity of the superficial epithelial structure in the intestine. Additionally, the concentrations of IL-6 and TNF-α in the colon tissues were significantly decreased in the EVs-treated mice. The abundances of the , , -, , and genera were increased in the gut after treatment with the milk-derived EVs. Additionally, the butyrate and acetate production were enriched in feces. In addition, 1659 genes were significantly down-regulated and 1981 genes were significantly up-regulated in the EVs-treated group. Meanwhile, 82 lncRNAs and 6 circRNAs were also differentially expressed. Overall, the milk-derived EVs could attenuate colitis through optimizing gut microbiota abundance and by manipulating intestinal gene expression, implying their application potential for colitis prevention.
牛源细胞外囊泡通过其携带的生物活性物质对新生哺乳动物发挥重要的组织特异性免疫保护功能。本研究旨在探讨牛源细胞外囊泡对葡聚糖硫酸钠（DSS）诱导的结肠炎模型的抗炎作用，并阐明其潜在的分子机制。将 60 只 C57BL/6 小鼠分为 NC 组（正常对照）、DSS 组（DSS+PBS）、DSS+LOW 组（DSS+1.5×10 p/g 细胞外囊泡）、DSS+MID 组（DSS+1.5×10 p/g 细胞外囊泡）和 DSS+HIG 组（DSS+1.0×10 p/g 细胞外囊泡）。采用组织病理学切片、肠道微生物组和肠道组织 RNA-Seq 全面评估减轻结肠炎的有益功能。结果显示，牛源细胞外囊泡有助于维持肠道表面上皮结构的完整性。此外，细胞外囊泡处理组小鼠结肠组织中 IL-6 和 TNF-α 浓度显著降低。处理后， 、 、 、 和 属的丰度在肠道中增加。此外，粪便中丁酸和乙酸的产生得到了富集。此外，细胞外囊泡处理组有 1659 个基因显著下调，1981 个基因显著上调。同时，有 82 个 lncRNA 和 6 个 circRNA 也表现出差异表达。总之，牛源细胞外囊泡通过优化肠道微生物群丰度和调控肠道基因表达来减轻结肠炎，这表明它们在预防结肠炎方面具有应用潜力。
Zotero 插件
