采用一测多评法及指纹图谱分析对 L. 进行质量评价。
Quality Assessment of L. Using Quantitative Analysis of Multi-Components by Single Marker and Fingerprint Analysis.
机构信息
State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
Molecules. 2022 Apr 20;27(9):2634. doi: 10.3390/molecules27092634.
The chromatographic fingerprint of 14 batches of L. samples were established in this study. The constituents of ten components in L. were determined using quantitative analysis of multi-components by single marker (QAMS) and the external standard method (ESM). Due to their stability and accessibility, chlorogenic acid and linarin were used as references to calculate the relative correction factors (RCFs) of apigenin-C-6,8-pentoside-hexoside, apigenin-C-6,8-di-pentoside, luteolin, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, chrysosplenetin B, and sbsinthin, based on high-performance liquid chromatography (HPLC). The value calculated by QAMS was consistent with that of the ESM, and the reproducibility of RCFs was found to be reliable. In conclusion, simultaneous determination of the ten components by the QAMS method and chromatographic fingerprint analysis were feasible and accurate in evaluating the quality of L. and can be used as reference in traditional Chinese medicine quality control.
本研究建立了 14 批 L. 的色谱指纹图谱。采用多成分单标记定量分析（QAMS）和外标法（ESM）测定了 10 个成分的含量。由于绿原酸和木犀草素的稳定性和易得性，将其用作参考，计算芹菜素-C-6,8-戊糖苷-己糖苷、芹菜素-C-6,8-二戊糖苷、木樨草素、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、桑辛素 B 和桑辛素的相对校正因子（RCF），基于高效液相色谱（HPLC）。QAMS 法计算的值与 ESM 法一致，且 RCF 的重现性可靠。总之，采用 QAMS 法同时测定这 10 个成分，并进行色谱指纹图谱分析，是评估 L. 质量的可行且准确的方法，可作为中药质量控制的参考。
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