School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
Molecules. 2019 Apr 17;24(8):1521. doi: 10.3390/molecules24081521.
() tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, -hydroxybenzyl alcohol, -hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) ( > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of tuber and giving references to promote the quality standards of herbal medicines.
天麻是一种有价值的草药，用于治疗许多疾病。建立天麻合理可行的质量评估方法的过程对于确保其临床安全性和疗效非常重要。本研究基于高效液相色谱（HPLC）指纹图谱分析，并结合多成分单标记定量分析（QAMS）方法，建立了一种有效的天麻质量综合评价方法。该方法同时测定了天麻素、-羟基苯甲醇、-羟基苯甲醛、parishin A、parishin B、parishin C 和 parishin E 这 7 种成分的含量，并以天麻素为参照标准。结果表明，QAMS 法与传统外标法（ESM）无显著性差异（>0.05，RSD<4.79%），提示 QAMS 法是一种可靠、方便的多成分含量测定方法，特别是在缺乏对照品的情况下。综上所述，该策略有助于简化天麻质量控制过程，为促进草药质量标准的制定提供参考。
