College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.
Zhejiang Yangtze Delta Region Pharmaceutical Technology Research Park, Hangzhou 310014, China.
Molecules. 2022 Apr 23;27(9):2724. doi: 10.3390/molecules27092724.
var. (Franch.) Hara is a perennial herb belonging to the Trilliaceae family. Ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) was used to detect the composition of different fractions of var. leaves. Meanwhile, the extracts of different fractions were evaluated for their cytotoxic activities against four selected human cancer cell lines and one human normal epithelial cell line based on the MTT assay method. Multivariate statistical analysis was performed to screen differential compounds and to analyze the distributions between different fractions. Finally, more than 60 compounds were obtained and identified from the different fractions of var. leaves, and the chloroform and n-butanol extracts showed significant cytotoxic effects on these four cancer cells. Several compounds were preliminarily identified from different fractions, including 36 steroidal saponins, 11 flavonoids, 10 ceramides, 8 lipids, 6 organic acids, and 8 other compounds. Various compounds were screened out as different chemical components of different fractions, which were considered as a potential substance basis for the cytotoxicity of var. leaves.
变叶木（Franch.）Hara 是苦苣苔科的一种多年生草本植物。采用超高效液相色谱四极杆飞行时间质谱（UPLC/Q-TOF MS）检测变叶木叶不同部位的成分。同时，根据 MTT 法，评估不同部位提取物对四种人癌细胞系和一种人正常上皮细胞系的细胞毒性活性。采用多元统计分析筛选差异化合物，并分析不同部位之间的分布。最后，从变叶木叶的不同部位中获得并鉴定了 60 多种化合物，氯仿和正丁醇提取物对这四种癌细胞表现出显著的细胞毒性作用。从不同部位初步鉴定了几种化合物，包括 36 种甾体皂苷、11 种黄酮类化合物、10 种神经酰胺、8 种脂类、6 种有机酸和 8 种其他化合物。从不同部位筛选出各种化合物作为不同部位的不同化学组成部分，这被认为是变叶木叶细胞毒性的潜在物质基础。
