Smart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung 25451, Korea.
Division of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology (UST), Daejeon 34113, Korea.
Molecules. 2022 Apr 25;27(9):2751. doi: 10.3390/molecules27092751.
Black soybean has been used in traditional medicine to treat inflammatory diseases, cancer, and diabetes and as a nutritional source since ancient times. We found that Korean black soybean cultivar A63 has more cyanidin-3--glucoside, (C3G), procyanidin B2 (PB2), and epicatechin (EPC) contents than other cultivars and has beneficial effects on cell viability and anti-oxidation. Given the higher concentration of anthocyanidins and their strong anti-oxidant activity, we predicted that A63 extract could relieve inflammatory disease symptoms, including those of atopic dermatitis (AD). Here, we evaluated the anti-AD activity of A63 extract in an oxazolone (OXA)-induced mouse model. A63 extract treatment significantly reduced epidermal thickness and inflammatory cell infiltration, downregulated the expression of AD gene markers, including Interleukin (IL)-4 and IL-5, and restored damaged skin barrier tissues. Furthermore, A63 extract influenced the activation of the signal transducer and activator of transcription (STAT) 3 and STAT6, extracellular regulatory kinase (ERK), and c-Jun N-terminal kinase (JNK) signaling pathways, which play a crucial role in the development of AD. Altogether, our results suggest that A63 can ameliorate AD-like skin inflammation by inhibiting inflammatory cytokine production and STAT3/6 and Mitogen-activated protein kinase (MAPK) signaling and restoring skin barrier function.
黑豆自古以来就被传统医学用于治疗炎症性疾病、癌症和糖尿病，并作为营养来源。我们发现，韩国黑豆品种 A63 的矢车菊素-3-葡萄糖苷（C3G）、原花青素 B2（PB2）和表儿茶素（EPC）含量高于其他品种，对细胞活力和抗氧化具有有益作用。鉴于花色苷浓度较高且抗氧化活性较强，我们预测 A63 提取物可以缓解炎症性疾病症状，包括特应性皮炎（AD）。在这里，我们评估了 A63 提取物在氧化氮酮（OXA）诱导的小鼠模型中对 AD 的治疗作用。A63 提取物处理可显著降低表皮厚度和炎症细胞浸润，下调 AD 基因标志物（包括白细胞介素（IL）-4 和 IL-5）的表达，并修复受损的皮肤屏障组织。此外，A63 提取物影响信号转导和转录激活因子（STAT）3 和 STAT6、细胞外调节激酶（ERK）和 c-Jun N-末端激酶（JNK）信号通路的激活，这些信号通路在 AD 的发展中起着关键作用。总的来说，我们的结果表明，A63 通过抑制炎症细胞因子的产生和 STAT3/6 和丝裂原活化蛋白激酶（MAPK）信号通路以及恢复皮肤屏障功能来改善 AD 样皮肤炎症。
