College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
National Coarse Cereals Engineering Research Center, Daqing 163319, China.
Molecules. 2022 Apr 27;27(9):2783. doi: 10.3390/molecules27092783.
Accumulating attention has been focused on resistant starch (RS) due to its blood-lipid-lowering activities. However, reports on the potential bioactivities of RS for preventing hyperlipidemia acute pancreatitis (HLAP) are limited. Therefore, in this study, an acute pancreatitis model was set up by feeding a hyperlipidemia diet to rats, and subsequently evaluating the anti-HLAP effect of RS in kidney beans. The results show that the IL-6, IL-1β, and TNF-α of serum in each RS group were decreased by 18.67-50.00%, 7.92-22.89%, and 8.06-34.04%, respectively, compared with the model group (MOD). In addition, the mRNA expression of tight junction protein ZO-1, occludin, and antibacterial peptides CRAMP and DEFB1 of rats in each RS group increased by 26.43-60.07%, 229.98-279.90%, 75.80-111.20%, and 77.86-109.07%, respectively. The height of the villi in the small intestine and the thickness of the muscle layer of rats were also increased, while the depth of the crypt decreased. The present study indicates that RS relieves intestinal inflammation, inhibits oxidative stress, and prevents related intestinal barrier damage. These results support the supplementation of RS as an effective nutritional intervention for HLAP and associated intestinal injury.
由于抗性淀粉 (RS) 具有降低血液脂质的作用，因此人们越来越关注它。然而，关于 RS 预防高脂血症性急性胰腺炎 (HLAP) 的潜在生物活性的报道有限。因此，在本研究中，通过给大鼠喂食高脂血症饮食建立了急性胰腺炎模型，随后评估了红芸豆 RS 的抗 HLAP 作用。结果表明，与模型组 (MOD) 相比，各 RS 组大鼠血清中的白细胞介素-6 (IL-6)、白细胞介素-1β (IL-1β) 和肿瘤坏死因子-α (TNF-α) 分别降低了 18.67%-50.00%、7.92%-22.89%和 8.06%-34.04%。此外，各 RS 组大鼠紧密连接蛋白 ZO-1、occludin 和抗菌肽 CRAMP 和 DEFB1 的 mRNA 表达分别增加了 26.43%-60.07%、229.98%-279.90%、75.80%-111.20%和 77.86%-109.07%。大鼠小肠绒毛高度和肌层厚度增加，隐窝深度降低。本研究表明，RS 可缓解肠道炎症，抑制氧化应激，防止相关肠道屏障损伤。这些结果支持补充 RS 作为 HLAP 及相关肠道损伤的有效营养干预措施。
