Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Korea.
Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si 17104, Korea.
Molecules. 2022 Apr 27;27(9):2795. doi: 10.3390/molecules27092795.
Nanoscience is a multidisciplinary skill with elucidated nanoscale particles and their advantages in applications to various fields. Owing to their economical synthesis, biocompatible nature, and widespread biomedical and environmental applications, the green synthesis of metal nanoparticles using medicinal plants has become a potential research area in biomedical research and functional food formulations. (GP) has been extensively used in traditional Chinese medicine to cure several diseases, including diabetes mellitus (DM). This is the first study in which we examined the efficacy of gold nanoparticles (GP-AuNPs) against obesity and related inflammation. GP extract was used as a capping agent to reduce Au to Au to form stable gold nanoparticles. The nanoparticles were characterized by using UV-VIS spectroscopy, and TEM images were used to analyze morphology. In contrast, the existence of the functional group was measured using FTIR, and size and shape were examined using XRD analysis. In vitro analysis on GP-AuNPs was nontoxic to RAW 264.7 cells and 3T3-L1 cells up to a specific concentration. It significantly decreased lipid accumulation in 3T3-L1 obese and reduced NO production in Raw 264.7 macrophage cells. The significant adipogenic genes PPARγ and CEPBα and a major pro-inflammatory cytokine TNF-α expression were quantified using RT-PCR. The GP-AuNPs decreased the face of these genes remarkably, revealing the antiadipogenic and anti-inflammatory activity of our synthesized GP-AuNPs. This study represents thorough research on the antiobesity effect of gold nanoparticles synthesized using a green approach and the efficacy instead of related inflammatory responses.
纳米科学是一门多学科的技能，阐明了纳米级粒子及其在各个领域应用中的优势。由于其经济合成、生物相容性以及广泛的生物医学和环境应用，使用药用植物进行金属纳米粒子的绿色合成已成为生物医学研究和功能性食品配方中的一个潜在研究领域。(GP)在传统中药中被广泛用于治疗多种疾病，包括糖尿病（DM）。这是我们首次研究金纳米粒子（GP-AuNPs）对肥胖和相关炎症的疗效。GP 提取物被用作封端剂，将 Au 还原为 Au 以形成稳定的金纳米粒子。使用紫外-可见分光光度计对纳米粒子进行了表征，并使用 TEM 图像分析了形貌。相比之下，使用 FTIR 测量了官能团的存在，使用 XRD 分析测量了尺寸和形状。在体外分析中，GP-AuNPs 在特定浓度下对 RAW 264.7 细胞和 3T3-L1 细胞没有毒性。它显著减少了 3T3-L1 肥胖细胞中的脂质积累，并降低了 Raw 264.7 巨噬细胞中的 NO 产生。使用 RT-PCR 定量了显著的脂肪生成基因 PPARγ和 CEPBα和主要的促炎细胞因子 TNF-α的表达。GP-AuNPs 显著降低了这些基因的表达，显示出我们合成的 GP-AuNPs 的抗脂肪生成和抗炎活性。这项研究代表了使用绿色方法合成的金纳米粒子的抗肥胖作用及其对相关炎症反应的疗效的全面研究。
