Nakano T, Kanakura Y, Nakahata T, Matsuda H, Kitamura Y
Fed Proc. 1987 Apr;46(5):1920-3.
Genetically mast cell-deficient W/Wv mice are useful for the analysis of mast cell biology, especially as recipients of bone marrow cells and skin pieces. Inasmuch as suspension and clonal cultures of mast cells have been developed, we combined these in vivo and in vitro systems. Suspension-cultured mast cells had morphological and biochemical characteristics similar to those of mucosal mast cells (MMC). However, i.p. injection of such cultured mast cells gave rise to development of cells with characteristics similar to those of connective tissue mast cells (CTMC). When peritoneal cells of normal +/+ mice were cultured in methylcellulose, pure mast cell colonies appeared. Cells from individual mast cell colonies were divided and injected into the skin and stomach wall of W/Wv mice; CTMC developed in the skin and MMC in the stomach mucosa. This indicates the presence of a common precursor for CTMC and MMC. Morphology of such bipotent mast cell precursors was studied by using micromanipulation. About 4% of morphologically identifiable peritoneal mast cells may function as the bipotent precursors. Although W/Wv mice showed a defect in resistance against ixodid ticks, injection of suspension-cultured mast cells normalized the defect. The four examples mentioned above indicate that combinations of in vivo and in vitro systems increase the usefulness of W/Wv mice.
基因性肥大细胞缺陷的W/Wv小鼠对于肥大细胞生物学分析很有用，特别是作为骨髓细胞和皮肤碎片的受体。鉴于已经建立了肥大细胞的悬浮培养和克隆培养，我们将这些体内和体外系统结合起来。悬浮培养的肥大细胞具有与黏膜肥大细胞（MMC）相似的形态和生化特征。然而，腹腔注射这种培养的肥大细胞会导致出现具有与结缔组织肥大细胞（CTMC）相似特征的细胞。当正常+/+小鼠的腹腔细胞在甲基纤维素中培养时，会出现纯肥大细胞集落。将单个肥大细胞集落的细胞分离并注射到W/Wv小鼠的皮肤和胃壁中；在皮肤中形成CTMC，在胃黏膜中形成MMC。这表明CTMC和MMC存在共同的前体。通过显微操作研究了这种双能肥大细胞前体的形态。大约4%形态可识别的腹腔肥大细胞可能作为双能前体发挥作用。尽管W/Wv小鼠在抵抗硬蜱方面存在缺陷，但注射悬浮培养的肥大细胞可使该缺陷恢复正常。上述四个例子表明，体内和体外系统的结合增加了W/Wv小鼠的实用性。
