Feng Jingyu, Lv Weixing, Xu Jing, Huang Zhe, Rui Wenjing, Lei Xihong, Ju Xuehai, Li Zhifang
Beijing key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University (CAU), Haidian District, Yuanmingyuanxilu 2, Beijing 100193, China.
Beijing Agricultural Extention Station, Huixinxili 10, Changyang District, Beijing 100029, China.
Plants (Basel). 2022 Apr 27;11(9):1176. doi: 10.3390/plants11091176.
Understanding the impact of arbuscular mycorrhizal fungi (AMF) upon the nitrogen (N) uptake of tomato ( L.) plants is crucial for effectively utilizing these beneficial microorganisms in industrial hydroponic tomato production. Yet it remains unknown whether, besides fungal delivery, the AMF also affects N uptake via altered plant root growth or whether, together with changed N transporters expression of hosts, this impact is isolate-specific. We investigated tomato root architecture and the expression of , , and genes in roots inoculated with five isolates of , these collected from different geographical locations, under greenhouse conditions with nutritional solution in coconut coir production. Our results revealed that isolate-specific AMF inoculation strongly increased the root biomass, total root length, surface area, and volume. Linear relationships were found between the total root length and N accumulation in plants. Furthermore, expression levels of , , and were significantly up-regulated by inoculation with with isolate-specific. These results implied N uptake greater than predicted by root growth, and N transporters up-regulated by AMF symbiosis in an isolate-specific manner. Thus, an overlap in root biomass, architecture and expression of N transporters increase N acquisition in tomato plants in the symbiosis.
了解丛枝菌根真菌（AMF）对番茄（L.）植株氮素吸收的影响，对于在工业水培番茄生产中有效利用这些有益微生物至关重要。然而，除了真菌传递外，AMF是否还通过改变植物根系生长来影响氮素吸收，或者与宿主氮转运蛋白表达的变化一起，这种影响是否具有菌株特异性，仍然未知。我们在温室条件下，以椰子纤维为基质，用营养液培养番茄，研究了接种从不同地理位置收集的5种AMF菌株的番茄根系结构以及根系中、和基因的表达情况。我们的结果表明，菌株特异性的AMF接种显著增加了根生物量、总根长、表面积和体积。发现植株的总根长与氮积累之间存在线性关系。此外，接种特定菌株的AMF后，、和的表达水平显著上调。这些结果表明，氮素吸收大于根系生长所预测的，并且AMF共生以菌株特异性方式上调氮转运蛋白。因此，根生物量、结构和氮转运蛋白表达的重叠增加了共生状态下番茄植株对氮的获取。
