Méndez Tamara, Stappung Yazmina, Moya-León María A, Herrera Raúl
Instituto de Ciencias Biológicas, Universidad de Talca, Av. Lircay s/n, Talca 3465548, Chile.
Plants (Basel). 2022 Apr 28;11(9):1190. doi: 10.3390/plants11091190.
Arabinogalactan proteins (AGPs) are members of a family of proteins that play important roles in cell wall dynamics. AGPs from inclined pines were determined using JIM7, LM2, and LM6 antibodies, showing a higher concentration in one side of the stem. The accumulation of AGPs in xylem and cell wall tissues is enhanced in response to loss of tree stem verticality. The differential gene expression of AGPs indicates that these proteins could be involved in the early response to inclination and also trigger signals such as lignin accumulation, as well as thicken cell wall and lamella media to restore stem vertical growth. A subfamily member of AGPs, which is Fasciclin-like has been described in angiosperm species as inducing tension wood and in some gymnosperms. A search for gene sequences of this subfamily was performed on an RNA-seq library, where 12 sequences were identified containing one or two fasciclin I domains (FAS), named PrFLA1 to PrFLA12. Four of these sequences were phylogenetically classified in group A, where PrFLA1 and PrFLA4 are differentially expressed in tilted pine trees.
阿拉伯半乳聚糖蛋白（AGPs）是在细胞壁动态变化中发挥重要作用的一类蛋白质家族成员。使用JIM7、LM2和LM6抗体测定了倾斜松树中的AGPs，结果显示其在茎的一侧浓度较高。响应于树干垂直度的丧失，AGPs在木质部和细胞壁组织中的积累会增强。AGPs的差异基因表达表明，这些蛋白质可能参与对倾斜的早期响应，并触发诸如木质素积累等信号，以及加厚细胞壁和中层介质以恢复茎的垂直生长。AGPs的一个亚家族成员，即类成束蛋白，已在被子植物物种中被描述为诱导产生张力木，在一些裸子植物中也有发现。在一个RNA测序文库中对该亚家族的基因序列进行了搜索，鉴定出12个含有一个或两个成束蛋白I结构域（FAS）的序列，命名为PrFLA1至PrFLA12。其中四个序列在系统发育上被归类为A组，其中PrFLA1和PrFLA4在倾斜松树中差异表达。
