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诱导下部弯曲松树干中 PrMADS10 的表达：在改变细胞壁特性和木材解剖结构中的潜在作用。

Induction of PrMADS10 on the lower side of bent pine tree stems: potential role in modifying plant cell wall properties and wood anatomy.

机构信息

Instituto de Ciencias Biológicas, Universidad de Talca. Av. Lircay s/n, Talca, Chile.

Facultad de Ciencias Ambientales, Universidad Técnica Estatal de Quevedo, Quevedo, Ecuador.

出版信息

Sci Rep. 2019 Dec 12;9(1):18981. doi: 10.1038/s41598-019-55276-7.

DOI:10.1038/s41598-019-55276-7
PMID:31831838
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6908731/
Abstract

The molecular mechanisms underlying inclination responses in trees are unclear. In this study, we identified a MADS-box transcription factor differentially expressed early after inclination in the stems of Pinus radiata D. Don. PrMADS10 has a CDS of 582 bp and encodes a group II MADS-box transcription factor. We measured highest accumulation of this transcript on the lower side of inclined pine stems. In an effort to identify putative targets, we stably transformed Arabidopsis thaliana with a 35S::PrMADS10 construct. Transcriptome analysis revealed 1,219 genes differentially-expressed, with 690 and 529 genes up- and down-regulated respectively, when comparing the transgenic and wild-type. Differentially-expressed genes belong to different biological processes, but were enriched in cell wall remodeling and phenylpropanoid metabolic functions. Interestingly, lignin content was 30% higher in transgenic as compared to wild-type plants consistent with observed changes in gene expression. Differentially expressed transcription factors and phenylpropanoid genes were analyzed using STRING. Several MYB and NAC transcription factors showed interactions with genes of the phenylpropanoid pathway. Together, these results implicate PrMADS10 as a regulatory factor, triggering the expression of other transcription factors and genes involved in the synthesis of lignin.

摘要

树木倾斜反应的分子机制尚不清楚。本研究鉴定了在辐射松茎中倾斜后早期差异表达的一个 MADS-box 转录因子。PrMADS10 的 CDS 为 582bp，编码一个 II 组 MADS-box 转录因子。我们在倾斜松茎的下侧测量到该转录本的最高积累量。为了鉴定潜在的靶标，我们使用 35S::PrMADS10 构建体稳定转化拟南芥。转录组分析显示，与野生型相比，有 1219 个基因差异表达，其中分别有 690 个和 529 个基因上调和下调。差异表达的基因属于不同的生物学过程，但在细胞壁重塑和苯丙烷代谢功能中富集。有趣的是，与观察到的基因表达变化一致，转基因植物的木质素含量比野生型高 30%。使用 STRING 分析差异表达的转录因子和苯丙烷基因。几个 MYB 和 NAC 转录因子与苯丙烷途径的基因表现出相互作用。总之，这些结果表明 PrMADS10 作为一个调节因子，触发了其他与木质素合成相关的转录因子和基因的表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/0a1a1a7cb79a/41598_2019_55276_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/1d9a64d0eb76/41598_2019_55276_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/9064ee66912c/41598_2019_55276_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/8af5c4a2c9b8/41598_2019_55276_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/83ff97e25732/41598_2019_55276_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/32f636549c71/41598_2019_55276_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/7a343de77837/41598_2019_55276_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/0a1a1a7cb79a/41598_2019_55276_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/1d9a64d0eb76/41598_2019_55276_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/9064ee66912c/41598_2019_55276_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/8af5c4a2c9b8/41598_2019_55276_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/83ff97e25732/41598_2019_55276_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/32f636549c71/41598_2019_55276_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/7a343de77837/41598_2019_55276_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f570/6908731/0a1a1a7cb79a/41598_2019_55276_Fig7_HTML.jpg

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