• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

MIF/SCL3A2 耗竭通过 AKT/GSK-3β 通路和细胞铁死亡抑制结直肠癌细胞的增殖和转移。

MIF/SCL3A2 depletion inhibits the proliferation and metastasis of colorectal cancer cells via the AKT/GSK-3β pathway and cell iron death.

机构信息

Department of Pathology, Nanfang Hospital and School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Department of Pathology, Shenzhen Longgang Central Hospital, Shenzhen, China.

出版信息

J Cell Mol Med. 2022 Jun;26(12):3410-3422. doi: 10.1111/jcmm.17352. Epub 2022 May 13.

DOI:10.1111/jcmm.17352
PMID:35567291
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9189354/
Abstract

This study investigated the mechanisms of migration inhibitory factor (MIF) and solute carrier family 3 member 2 (SLC3A2) in colorectal cancer progression. The levels of MIF and SLC3A2 expression in cells were measured by RT-qPCR. SW480 and SW620 cells were transfected with sh-MIF and sh-SLC3A2, respectively. MIF, SLC3A2, GPX4, E-cadherin and N-cadherin expression were detected by immunofluorescence (IF). CCK8 and Transwell assays were performed to detect cell proliferation and migration. Co-immunoprecipitation (CoIP) was used to measure the binding activity of MIF and SLC3A2. Finally, a nude mouse tumorigenicity assay was used to confirm the functions of MIF and SLC3A2 in colorectal cancer. Results showed that the levels of MIF and SLC3A2 expression were up-regulated in colorectal cancer cells. Inhibition of MIF or SLC3A2 expression prevented cell proliferation, migration, epithelial-mesenchymal transition (EMT) and invasion. In addition, knockdown of MIF and SLC3A2 promoted iron death in SW480 and SW620 cells. CoIP results showed that MIF and SLC3A2 directly interact with each other. Knockdown of both MIF and SLC3A2 inhibited tumour growth and metastasis via the AKT/GSK-3β pathway in vivo. The Akt/GSK-3β pathway was found to participate in regulating MIF and SLC3A2 both in vivo and in vitro. MIF and SLC3A2 might be potential biomarkers for monitoring the treatment of colorectal cancer.

摘要

本研究探讨了迁移抑制因子（MIF）和溶质载体家族 3 成员 2（SLC3A2）在结直肠癌进展中的作用机制。采用 RT-qPCR 检测细胞中 MIF 和 SLC3A2 表达水平。分别用 sh-MIF 和 sh-SLC3A2 转染 SW480 和 SW620 细胞。采用免疫荧光（IF）检测 MIF、SLC3A2、GPX4、E-钙黏蛋白和 N-钙黏蛋白的表达。通过 CCK8 和 Transwell 实验检测细胞增殖和迁移。采用 co-immunoprecipitation（CoIP）检测 MIF 和 SLC3A2 的结合活性。最后，通过裸鼠肿瘤生成实验证实 MIF 和 SLC3A2 在结直肠癌中的作用。结果表明，结直肠癌细胞中 MIF 和 SLC3A2 的表达水平上调。抑制 MIF 或 SLC3A2 的表达可阻止细胞增殖、迁移、上皮-间充质转化（EMT）和侵袭。此外，下调 MIF 和 SLC3A2 可促进 SW480 和 SW620 细胞铁死亡。CoIP 结果表明，MIF 和 SLC3A2 直接相互作用。体内敲低 MIF 和 SLC3A2 通过 AKT/GSK-3β 通路抑制肿瘤生长和转移。体内外研究均发现 Akt/GSK-3β 通路参与调节 MIF 和 SLC3A2。MIF 和 SLC3A2 可能是监测结直肠癌治疗的潜在生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/f265b425d080/JCMM-26-3410-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/0186c4a95ed6/JCMM-26-3410-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/c7a36ff208f8/JCMM-26-3410-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/0e9ace0d5197/JCMM-26-3410-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/3ef3f73b0128/JCMM-26-3410-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/10e897bf6862/JCMM-26-3410-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/3301ef9d120e/JCMM-26-3410-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/f4d2bb999c46/JCMM-26-3410-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/f265b425d080/JCMM-26-3410-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/0186c4a95ed6/JCMM-26-3410-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/c7a36ff208f8/JCMM-26-3410-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/0e9ace0d5197/JCMM-26-3410-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/3ef3f73b0128/JCMM-26-3410-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/10e897bf6862/JCMM-26-3410-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/3301ef9d120e/JCMM-26-3410-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/f4d2bb999c46/JCMM-26-3410-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53aa/9189354/f265b425d080/JCMM-26-3410-g006.jpg

相似文献

1
MIF/SCL3A2 depletion inhibits the proliferation and metastasis of colorectal cancer cells via the AKT/GSK-3β pathway and cell iron death.MIF/SCL3A2 耗竭通过 AKT/GSK-3β 通路和细胞铁死亡抑制结直肠癌细胞的增殖和转移。
J Cell Mol Med. 2022 Jun;26(12):3410-3422. doi: 10.1111/jcmm.17352. Epub 2022 May 13.
2
Ponicidin inhibits pro-inflammatory cytokine TNF-α-induced epithelial-mesenchymal transition and metastasis of colorectal cancer cells via suppressing the AKT/GSK-3β/Snail pathway.蓬尼定通过抑制 AKT/GSK-3β/Snail 通路抑制促炎细胞因子 TNF-α 诱导的结直肠癌细胞上皮-间充质转化和转移。
Inflammopharmacology. 2019 Jun;27(3):627-638. doi: 10.1007/s10787-018-0534-5. Epub 2018 Sep 22.
3
PGC-1α Regulates Cell Proliferation and Invasion AKT/GSK-3β/β-catenin Pathway in Human Colorectal Cancer SW620 and SW480 Cells.PGC-1α 通过 AKT/GSK-3β/β-catenin 通路调控人结直肠癌细胞 SW620 和 SW480 的增殖和侵袭
Anticancer Res. 2020 Feb;40(2):653-664. doi: 10.21873/anticanres.13995.
4
CHIP functions as an oncogene by promoting colorectal cancer metastasis via activation of MAPK and AKT signaling and suppression of E-cadherin.CHIP 通过激活 MAPK 和 AKT 信号通路和抑制 E-钙黏蛋白来促进结直肠癌转移，从而发挥癌基因的作用。
J Transl Med. 2018 Jun 19;16(1):169. doi: 10.1186/s12967-018-1540-5.
5
PI3K/Akt/GSK-3β signal pathway is involved in P2X7 receptor-induced proliferation and EMT of colorectal cancer cells.PI3K/Akt/GSK-3β 信号通路参与 P2X7 受体诱导的结直肠癌细胞增殖和 EMT。
Eur J Pharmacol. 2021 May 15;899:174041. doi: 10.1016/j.ejphar.2021.174041. Epub 2021 Mar 15.
6
Nitidine chloride suppresses epithelial-to-mesenchymal transition in osteosarcoma cell migration and invasion through Akt/GSK-3β/Snail signaling pathway.氯化两面针碱通过Akt/GSK-3β/Snail信号通路抑制骨肉瘤细胞迁移和侵袭中的上皮-间质转化。
Oncol Rep. 2016 Aug;36(2):1023-9. doi: 10.3892/or.2016.4846. Epub 2016 Jun 2.
7
[MiR-451 regulates proliferation and migration of colorectal cells by targeting MIF].[微小RNA-451通过靶向巨噬细胞移动抑制因子调控结肠直肠细胞的增殖和迁移]
Zhonghua Zhong Liu Za Zhi. 2020 Apr 23;42(4):312-318. doi: 10.3760/cma.j.cn112152-20190924-00622.
8
CHN1 promotes epithelial-mesenchymal transition via the Akt/GSK-3β/Snail pathway in cervical carcinoma.CHN1 通过 Akt/GSK-3β/Snail 通路促进宫颈癌中的上皮-间质转化。
J Transl Med. 2021 Jul 8;19(1):295. doi: 10.1186/s12967-021-02963-7.
9
Elevated Expression of Zinc Finger Protein 703 Promotes Cell Proliferation and Metastasis through PI3K/AKT/GSK-3β Signalling in Oral Squamous Cell Carcinoma.锌指蛋白703的高表达通过PI3K/AKT/GSK-3β信号通路促进口腔鳞状细胞癌的细胞增殖和转移。
Cell Physiol Biochem. 2017;44(3):920-934. doi: 10.1159/000485360. Epub 2017 Nov 24.
10
Synergistic effects of CD44 and TGF-β1 through AKT/GSK-3β/β-catenin signaling during epithelial-mesenchymal transition in liver cancer cells.CD44与转化生长因子-β1在肝癌细胞上皮-间质转化过程中通过AKT/糖原合成酶激酶-3β/β-连环蛋白信号通路产生的协同作用。
Biochem Biophys Res Commun. 2016 Sep 2;477(4):568-574. doi: 10.1016/j.bbrc.2016.06.077. Epub 2016 Jun 16.

引用本文的文献

1
Epigenetically-Upregulated CD98 Shed Light on the Precancerous Diagnosis and Prognosis Prediction of Esophageal Cancer.表观遗传上调的CD98为食管癌的癌前诊断和预后预测提供线索。
Cancer Sci. 2025 Sep;116(9):2592-2606. doi: 10.1111/cas.70128. Epub 2025 Jun 30.
2
Development and validation of a disulfidptosis-related prognostic model for colorectal cancer using multi-omics analysis.基于多组学分析的结直肠癌二硫键连接性坏死相关预后模型的构建与验证
Discov Oncol. 2025 Mar 17;16(1):338. doi: 10.1007/s12672-025-02055-8.
3
Macrophage migration inhibitory factor in inflammasome formation and macrophage recruitment by cervical squamous cell carcinoma cells.

本文引用的文献

1
Co-Expression Effect of SLC7A5/SLC3A2 to Predict Response to Endocrine Therapy in Oestrogen-Receptor-Positive Breast Cancer.SLC7A5/SLC3A2 共表达对预测雌激素受体阳性乳腺癌内分泌治疗反应的影响。
Int J Mol Sci. 2020 Feb 19;21(4):1407. doi: 10.3390/ijms21041407.
2
SKA3 Promotes tumor growth by regulating CDK2/P53 phosphorylation in hepatocellular carcinoma.SKA3 通过调节肝癌细胞中 CDK2/P53 的磷酸化促进肿瘤生长。
Cell Death Dis. 2019 Dec 5;10(12):929. doi: 10.1038/s41419-019-2163-3.
3
Colorectal cancer.结直肠癌。
巨噬细胞移动抑制因子在宫颈鳞状细胞癌细胞形成炎性小体及募集巨噬细胞中的作用
Oncol Lett. 2025 Jan 28;29(4):164. doi: 10.3892/ol.2025.14910. eCollection 2025 Apr.
4
Therapeutic Opportunities in Breast Cancer by Targeting Macrophage Migration Inhibitory Factor as a Pleiotropic Cytokine.通过靶向巨噬细胞移动抑制因子这一多功能细胞因子探索乳腺癌的治疗新机遇
Breast Cancer (Auckl). 2024 Sep 6;18:11782234241276310. doi: 10.1177/11782234241276310. eCollection 2024.
5
Amino acid transporters within the solute carrier superfamily: Underappreciated proteins and novel opportunities for cancer therapy.溶质载体家族中的氨基酸转运蛋白：被低估的蛋白质和癌症治疗的新机会。
Mol Metab. 2024 Jun;84:101952. doi: 10.1016/j.molmet.2024.101952. Epub 2024 May 3.
6
Study on the Role and Mechanism of SLC3A2 in Tumor-Associated Macrophage Polarization and Bladder Cancer Cells Growth.SLC3A2在肿瘤相关巨噬细胞极化及膀胱癌细胞生长中的作用和机制研究
Technol Cancer Res Treat. 2024 Jan-Dec;23:15330338241246649. doi: 10.1177/15330338241246649.
7
Macrophage migration inhibitory factor (MIF) predicts survival in patients with clear cell renal cell carcinoma.巨噬细胞移动抑制因子（MIF）可预测透明细胞肾细胞癌患者的生存率。
J Pathol Clin Res. 2024 Mar;10(2):e12365. doi: 10.1002/2056-4538.12365.
8
Glutamine metabolism in tumor metastasis: Genes, mechanisms and the therapeutic targets.肿瘤转移中的谷氨酰胺代谢：基因、机制及治疗靶点
Heliyon. 2023 Oct 5;9(10):e20656. doi: 10.1016/j.heliyon.2023.e20656. eCollection 2023 Oct.
9
CD98 heavy chain as a prognostic biomarker and target for cancer treatment.CD98重链作为癌症治疗的预后生物标志物和靶点。
Front Oncol. 2023 Sep 26;13:1251100. doi: 10.3389/fonc.2023.1251100. eCollection 2023.
10
Macrophage migration inhibitory factor exacerbates asthmatic airway remodeling via dynamin-related protein 1-mediated autophagy activation.巨噬细胞移动抑制因子通过与动力相关蛋白 1 介导的自噬激活加重哮喘气道重塑。
Respir Res. 2023 Sep 6;24(1):216. doi: 10.1186/s12931-023-02526-y.
Lancet. 2019 Oct 19;394(10207):1467-1480. doi: 10.1016/S0140-6736(19)32319-0.
4
CD98hc (SLC3A2) sustains amino acid and nucleotide availability for cell cycle progression.CD98hc（SLC3A2）维持细胞周期进程所需的氨基酸和核苷酸的可用性。
Sci Rep. 2019 Oct 1;9(1):14065. doi: 10.1038/s41598-019-50547-9.
5
Inflammasomes in Colitis and Colorectal Cancer: Mechanism of Action and Therapies.炎症小体在结肠炎和结直肠癌中的作用机制及治疗策略。
Yale J Biol Med. 2019 Sep 20;92(3):481-498. eCollection 2019 Sep.
6
Transgelin 2 Promotes Paclitaxel Resistance, Migration, and Invasion of Breast Cancer by Directly Interacting with PTEN and Activating PI3K/Akt/GSK-3β Pathway.转胶蛋白 2 可通过与 PTEN 直接相互作用并激活 PI3K/Akt/GSK-3β 通路促进乳腺癌对紫杉醇的耐药性、迁移和侵袭。
Mol Cancer Ther. 2019 Dec;18(12):2457-2468. doi: 10.1158/1535-7163.MCT-19-0261. Epub 2019 Sep 5.
7
MiR-203a-3p regulates the biological behaviors of ovarian cancer cells through mediating the Akt/GSK-3β/Snail signaling pathway by targeting ATM.miR-203a-3p 通过靶向 ATM 调控 Akt/GSK-3β/Snail 信号通路对卵巢癌细胞的生物学行为进行调控。
J Ovarian Res. 2019 Jul 5;12(1):60. doi: 10.1186/s13048-019-0532-2.
8
SLC3A2/CD98hc, autophagy and tumor radioresistance: a link confirmed.溶质载体家族 3 成员 2/CD98 重链，自噬和肿瘤放射抵抗：被证实的关联。
Autophagy. 2019 Oct;15(10):1850-1851. doi: 10.1080/15548627.2019.1639302. Epub 2019 Jul 15.
9
Iron homeostasis and iron-regulated ROS in cell death, senescence and human diseases.铁稳态和铁调节的 ROS 在细胞死亡、衰老和人类疾病中的作用。
Biochim Biophys Acta Gen Subj. 2019 Sep;1863(9):1398-1409. doi: 10.1016/j.bbagen.2019.06.010. Epub 2019 Jun 20.
10
The immunobiology of MIF: function, genetics and prospects for precision medicine.MIF 的免疫生物学：功能、遗传学和精准医学的前景。
Nat Rev Rheumatol. 2019 Jul;15(7):427-437. doi: 10.1038/s41584-019-0238-2. Epub 2019 Jun 13.