兔卵的受精以及卵膜结构在多精受精阻断中的作用。
Fertilization of rabbit ova and the role of ovum investments in the block to polyspermy.
作者信息
McCulloh D H, Wall R J, Levitan H
出版信息
Dev Biol. 1987 Apr;120(2):385-91. doi: 10.1016/0012-1606(87)90241-7.
The rabbit ovum seldom becomes polyspermic despite the presence of supernumerary sperm with easy access to the ovum plasma membrane. The purpose of this study was to characterize the role of ovum investments in blocking polyspermy. Ova were inseminated with capacitated sperm in vitro and were fertilized. Early stages of development were normal. The incidence of polyspermy was determined by cytological examination of fixed ova. The incidence of polyspermy increased following removal of both the zona pellucida and corona radiata but not following removal of only the corona radiata. These results suggest that the failure of supernumerary sperm to penetrate the ovum plasma membrane is at least in part due to the presence of the zona pellucida.
尽管存在多余的精子且能轻易接触到卵质膜，但兔卵很少发生多精受精。本研究的目的是确定卵膜在阻止多精受精中的作用。将卵子在体外与获能精子受精。早期发育阶段正常。通过对固定卵子的细胞学检查确定多精受精的发生率。去除透明带和放射冠后，多精受精的发生率增加，但仅去除放射冠后则不会增加。这些结果表明，多余精子未能穿透卵质膜至少部分是由于透明带的存在。
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