用于血清和活细胞中γ-谷氨酰转肽酶检测的比率型荧光探针。
A ratiometric fluorescent probe for detection of γ-glutamyl transpeptidase in blood serum and living cells.
机构信息
Key Laboratory of Photochemical Conversion and Optoelectronic Materials and City U-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Key Laboratory of Photochemical Conversion and Optoelectronic Materials and City U-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China; School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 5;278:121325. doi: 10.1016/j.saa.2022.121325. Epub 2022 May 5.
γ-Glutamyl transpeptidase (GGT) is one of the biomarker of cancer, hepatitis, and numerous other diseases. The accurate analysis of GGT is useful for the early diagnosis of these diseases. In this work, Probe 1, a ratiometric fluorescent probe based on 2,3,5,6-tetrafluoroterephthalonitrile, was designed for GGT detection. The results indicated that Probe 1 can sensitively and selectively detect GGT in phosphate buffered solution and complex biological systems (e.g., blood serum). Furthermore, Probe 1 has been successfully applied for ratiometric imaging of GGT in cancer cells and normal cells.
γ-谷氨酰转肽酶（GGT）是癌症、肝炎和许多其他疾病的生物标志物之一。准确分析 GGT 有助于这些疾病的早期诊断。在这项工作中，设计了基于 2,3,5,6-四氟对苯二甲腈的比率型荧光探针 Probe 1，用于 GGT 的检测。结果表明，Probe 1 可以在磷酸盐缓冲溶液和复杂的生物体系（如血清）中灵敏且选择性地检测 GGT。此外，Probe 1 已成功应用于癌细胞和正常细胞中 GGT 的比率成像。
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