College of Materials Science & Engineering, State Key Laboratory of Luminescent Materials & Devices, South China University of Technology, Guangzhou 510640, China.
College of Materials Science & Engineering, State Key Laboratory of Luminescent Materials & Devices, South China University of Technology, Guangzhou 510640, China.
Biosens Bioelectron. 2016 Nov 15;85:317-323. doi: 10.1016/j.bios.2016.05.036. Epub 2016 May 10.
γ-Glutamyl transpeptidase (GGT) plays crucial roles in some physiological processes. Herein a turn-on fluorescent probe for γ-glutamyl transpeptidase (GGT) assay based on aggregation-induced-emission (AIE) effect and the enzyme-induced transformation of hydrophilicity to hydrophobicity has been developed by functionalizing tetraphenylethylene (TPE) derivative with two γ-glutamyl amide groups, which simultaneously work as recognition units and hydrophilic groups. When the γ-glutamyl amide groups are cleaved through GGT enzymatic reaction, the hydrophobic reaction product readily aggregate and correspondingly strong blue fluorescence can be observed, as a result of activated AIE process. By virtue of the probe's good solubility in totally aqueous solution, high sensitivity and excellent photostability, the probe can be employed to detect GGT level in human serum samples. Furthermore, the probe can be used for imaging endogenous GGT in living A2780 cells. Hence, the probe holds great promise for acting as a convenient one-step straightforward assay for GGT detection in diagnostic-related applications, and also it could provide a useful approach for conducting pathological analysis for diseases involving GGT.
γ-谷氨酰转肽酶（GGT）在一些生理过程中发挥着关键作用。在此，通过将两个γ-谷氨酰酰胺基官能化到四苯乙烯（TPE）衍生物上，设计了一种基于聚集诱导发射（AIE）效应和酶诱导的亲水性向疏水性转变的用于γ-谷氨酰转肽酶（GGT）分析的荧光探针，其同时作为识别单元和亲水性基团。当γ-谷氨酰酰胺基通过 GGT 酶反应被切断时，疏水性反应产物容易聚集，并且由于 AIE 过程被激活，可以观察到相应的强蓝色荧光。由于探针在完全水性溶液中具有良好的溶解性、高灵敏度和优异的光稳定性，因此可以用于检测人血清样品中的 GGT 水平。此外，该探针可用于在活的 A2780 细胞中对内源性 GGT 进行成像。因此，该探针有望成为诊断相关应用中 GGT 检测的一种方便的一步式直接测定方法，也为涉及 GGT 的疾病的病理分析提供了一种有用的方法。
