Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing, China.
Luminescence. 2024 Aug;39(8):e4860. doi: 10.1002/bio.4860.
A sensitive benzothiazole fluorescent probe (PBZO) for the detection of γ-glutamyl transpeptidase (GGT) activity was developed. Based on the enzymatic hydrolysis of peptide bonds by glutamyl transpeptidase, it can be specifically recognized by PBZO. The PBZO has a good linear relationship with different gradients of GGT activity at the emission wavelength of 560 nm, the Stokes shift reached 215 nm, and the detection limit of GGT activity is 0.1644 U/ml. With the increase of GGT concentration in the probe solution, the color of the solution gradually changed from orange to dark yellow under the 365 nm UV lamp. The same color change was also observed on the probe test paper. In addition, there is a linear relationship between the GGT activity and the R-value of the probe solution. More importantly, the probe has a good recovery rate in serum. Therefore, this probe can be used as a convenient tool for detecting GGT activity.
开发了一种用于检测γ-谷氨酰转肽酶（GGT）活性的灵敏苯并噻唑荧光探针（PBZO）。基于谷氨酰转肽酶对肽键的酶解，可以特异性地被 PBZO 识别。在发射波长为 560nm 时，PBZO 与不同梯度的 GGT 活性具有良好的线性关系，Stokes 位移达到 215nm，GGT 活性的检测限为 0.1644U/ml。随着探针溶液中 GGT 浓度的增加，溶液在 365nm 紫外灯下的颜色逐渐从橙色变为深黄色。在探针试纸上也观察到相同的颜色变化。此外，探针溶液的 GGT 活性与 R 值之间存在线性关系。更重要的是，该探针在血清中有良好的回收率。因此，该探针可用作检测 GGT 活性的便捷工具。
