Ma Haihua, Sun Jizhou, Zhang Yuan, Bian Chao, Xia Shanhong, Zhen Tong
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China; Graduate University of Chinese Academic of Sciences, Beijing 100190, PR China; College of Information Science and Engineering, Henan University of Technology, Zhengzhou 450001, PR China; Key Laboratory of Grain Information Processing and Control of Ministry of Education, Henan University of Technology, Zhengzhou 450001, PR China.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, PR China.
Biosens Bioelectron. 2016 Jun 15;80:222-229. doi: 10.1016/j.bios.2016.01.063. Epub 2016 Jan 28.
Gold nanoparticles (AuNPs) embedded in chitosan (CHI) film, well-dispersed and smaller in size (about 10 nm), were fabricated by one-step electrodeposion on Au microelectrode in solution containing chitosan and chloride trihydrate. The nano-structure CHI-AuNPs composite film offers abundant amine groups, good conductivity, excellent biocompatibility and stability for antibody immobilization. The combination of aflatoxin B1 (AFB1) with immobilized antibody introduces a barrier to electron transfer, resulting in current decreasement. The morphologies and characterizations of modified microelectrodes were investigated by scanning electron microscope (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and Fourier transform infrared spectroscopy (FT-IR). The proposed non-enzyme and label-free immunosensor exhibited high sensitive amperometric response to AFB1 concentration in two linear ranges of 0.1 to 1 ng mL(-1) and 1 to 30 ng mL(-1), with the detection limit of 0.06 ng mL(-1) (S/N=3). The immunoassay was also applied for analysis of maize samples spiked with AFB1. Considering the sample extraction procedure, the linear range and limit of detection were assessed to be 1.6-16 ng mL(-1) and 0.19 ng mL(-1) respectively. The simple method showed good fabrication controllability and reproducibility for immunosensor design.
通过在含有壳聚糖和三水合氯化物的溶液中在金微电极上进行一步电沉积，制备了嵌入壳聚糖（CHI）膜中的金纳米颗粒（AuNPs），其分散良好且尺寸较小（约10 nm）。纳米结构的CHI-AuNPs复合膜具有丰富的胺基、良好的导电性、优异的生物相容性以及用于抗体固定的稳定性。黄曲霉毒素B1（AFB1）与固定化抗体的结合会引入电子转移障碍，导致电流降低。通过扫描电子显微镜（SEM）、循环伏安法（CV）、电化学阻抗谱（EIS）和傅里叶变换红外光谱（FT-IR）对修饰微电极的形态和特性进行了研究。所提出的非酶无标记免疫传感器在0.1至1 ng mL⁻¹和1至30 ng mL⁻¹两个线性范围内对AFB1浓度表现出高灵敏的安培响应，检测限为0.06 ng mL⁻¹（S/N = 3）。该免疫分析方法还应用于添加了AFB1的玉米样品分析。考虑到样品提取过程，评估其线性范围和检测限分别为1.6 - 16 ng mL⁻¹和0.19 ng mL⁻¹。该简单方法在免疫传感器设计方面显示出良好的制备可控性和重现性。
Zotero 插件